Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA sequencing of circulating tumour cells implicates WNT signaling in pancreatic cancer metastasis

ABSTRACT: RNA sequencing of circulating tumour cells implicates WNT signaling in pancreatic cancer metastasis

PROVIDER: PRJNA173017 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR543324.fastq.gz	Fastqsanger.gz
	SRR543325.fastq.gz	Fastqsanger.gz
	SRR543326.fastq.gz	Fastqsanger.gz
	SRR543327.fastq.gz	Fastqsanger.gz
	SRR543328.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 44

Similar Datasets

RNA sequencing of circulating tumour cells implicates WNT signaling in pancreatic cancer metastasis

Project description:This SuperSeries is composed of the following subset Series: GSE40171: RNA sequencing of circulating tumour cells implicates WNT signaling in pancreatic cancer metastasis (mouse data) GSE40174: RNA sequencing of circulating tumour cells implicates WNT signaling in pancreatic cancer metastasis (human data) Refer to individual Series

2012-08-17 | E-GEOD-40176 | biostudies-arrayexpress

RNA sequencing of circulating tumour cells implicates WNT signaling in pancreatic cancer metastasis (human data)

Project description:Circulating tumour cells (CTCs) shed into blood from primary cancers include putative precursors that initiate distal metastases. While these cells are extraordinarily rare, they may identify cellular pathways contributing to the blood-borne dissemination of cancer. Here, we adapted a microfluidic device for efficient capture of CTCs from an endogenous mouse pancreatic cancer model and subjected CTCs to single-molecule RNA sequencing, identifying Wnt2 as enriched in CTCs. Expression of Wnt2 in pancreatic cancer cells suppresses anoikis, enhances anchorage-independent sphere formation, and increases metastatic propensity in vivo. The effect of Wnt2 is correlated with fibronectin upregulation, and it is mediated in part through non-canonical Wnt signaling and suppressed by inhibition of the Map3k7 (Tak1) kinase, an integrator of Wnt, BMP and TGF-beta signaling. In humans, formation of non-adherent tumour spheres by pancreatic cancer cells is associated with upregulation of multiple Wnt genes, and pancreatic CTCs revealed significant enrichment for non-canonical Wnt signaling in 5 of 11 cases. Thus, molecular analysis of CTCs may identify novel therapeutic targets to prevent the distal spread of cancer. Expression profiling of circulating tumor cells in human pancreatic cancer patients support a hypothesis that WNT signaling plays a role in pancreatic cancer metastasis.

2012-08-17 | E-GEOD-40174 | biostudies-arrayexpress

RNA sequencing of circulating tumour cells implicates WNT signaling in pancreatic cancer metastasis (mouse data)

Project description:Circulating tumour cells (CTCs) shed into blood from primary cancers include putative precursors that initiate distal metastases. While these cells are extraordinarily rare, they may identify cellular pathways contributing to the blood-borne dissemination of cancer. Here, we adapted a microfluidic device for efficient capture of CTCs from an endogenous mouse pancreatic cancer model and subjected CTCs to single-molecule RNA sequencing, identifying Wnt2 as enriched in CTCs. Expression of Wnt2 in pancreatic cancer cells suppresses anoikis, enhances anchorage-independent sphere formation, and increases metastatic propensity in vivo. The effect of Wnt2 is correlated with fibronectin upregulation, and it is mediated in part through non-canonical Wnt signaling and suppressed by inhibition of the Map3k7 (Tak1) kinase, an integrator of Wnt, BMP and TGF-beta signaling. In humans, formation of non-adherent tumour spheres by pancreatic cancer cells is associated with upregulation of multiple Wnt genes, and pancreatic CTCs revealed significant enrichment for non-canonical Wnt signaling in 5 of 11 cases. Thus, molecular analysis of CTCs may identify novel therapeutic targets to prevent the distal spread of cancer. Expression profiling of primary tumor, circulating tumor cells and ascites in a mouse model of pancreatic cancer suggested WNT signaling plays a role in pancreatic cancer metastasis. Induction of Wnt2 signaling in mouse pancreatic NB508 cells supported the hypothesis.

2012-08-17 | E-GEOD-40171 | biostudies-arrayexpress

RNA sequencing of circulating tumour cells implicates WNT signaling in pancreatic cancer metastasis (human data)

2012-08-17 | GSE40174 | GEO

RNA sequencing of circulating tumour cells implicates WNT signaling in pancreatic cancer metastasis (mouse data)

2012-08-17 | GSE40171 | GEO

RNA sequencing of pancreatic circulating tumour cells implicates WNT signalling in metastasis.

Project description:Circulating tumour cells (CTCs) shed into blood from primary cancers include putative precursors that initiate distal metastases. Although these cells are extraordinarily rare, they may identify cellular pathways contributing to the blood-borne dissemination of cancer. Here, we adapted a microfluidic device for efficient capture of CTCs from an endogenous mouse pancreatic cancer model and subjected CTCs to single-molecule RNA sequencing, identifying Wnt2 as a candidate gene enriched in CTCs. Expression of WNT2 in pancreatic cancer cells suppresses anoikis, enhances anchorage-independent sphere formation, and increases metastatic propensity in vivo. This effect is correlated with fibronectin upregulation and suppressed by inhibition of MAP3K7 (also known as TAK1) kinase. In humans, formation of non-adherent tumour spheres by pancreatic cancer cells is associated with upregulation of multiple WNT genes, and pancreatic CTCs revealed enrichment for WNT signalling in 5 out of 11 cases. Thus, molecular analysis of CTCs may identify candidate therapeutic targets to prevent the distal spread of cancer.

| S-EPMC3408856 | biostudies-literature

RNA sequencing of circulating tumour cells implicates WNT signaling in pancreatic cancer metastasis (human data)

| S-ECPF-GEOD-40174 | biostudies-other

Detecting circulating tumour DNA from gestational trophoblastic tumour using common SNPs

Project description:Gestational tumour diagnosis using circulating tumour DNA

| PRJEB49976 | ENA

RNA sequencing of circulating tumour cells implicates WNT signaling in pancreatic cancer metastasis

Project description:This SuperSeries is composed of the SubSeries listed below.

2012-08-17 | GSE40176 | GEO

Pancreatic Circulating Tumour Cell Profiling Identifies LIN28B as a Metastasis Driver and Drug Target

Project description:Pancreatic Circulating Tumour Cell Profiling Identifies LIN28B as a Metastasis Driver and Drug Target

| PRJNA604022 | ENA