Project description:Single channel custom oligonucleotide array of 147 microRNAs to detect changes in expression of a lgl-hypomorph mutant versus wild-type 0, 3, and 5 day old 3rd instar lgl-hypomorph larvae were compared to 0 day 3rd instar wild-type larvae to test for differences in microRNA expression
Project description:mRNA microarray of Drosophila wing imaginal discs or brain complexes from lgl27S3/lglE2S31 (lgl-null), scrib1/scrib2 or wild-type 3rd instar larvae RNA was isolated from 20 pairs of wing discs/brain complexes per sample (i.e 20 individuals). Samples were from 5 day AED wildtype and 9 day AED mutant tissue
Project description:mRNA microarray of Drosophila wing imaginal discs or brain complexes from lgl27S3/lglE2S31 (lgl-null), scrib1/scrib2 or wild-type 3rd instar larvae RNA was isolated from 20 pairs of wing discs/brain complexes per sample (i.e 20 individuals). Samples were from 5 day AED wildtype and 9 day AED mutant tissue Samples were prepared in triplicate from lgl27S3/lglE2S31 (lgl-null) and scrib1/scrib2 samples and in duplicate from the wild-type 3rd instar larvae
Project description:mRNA microarray of Drosophila melanogaster extratced from cephalic complexes of lgl27S3/lglE2S31 (lgl-null) and FRT82B (wild-type) 3rd instar larvae
Project description:Affymetrix microarray to detect changes in gene expression between lgl27S3/lglE2S31 and FRT82B larvae 4-day old 3rd instar lgl27S3/lglE2S31 larvae were compared to 0-day old 3rd instar FRT82B larvae
Project description:Total protein was isolated from the trachea and midgut of D. melanogaster 3rd instar larvae and analysed by shotgun proteomics in order to identify the putative carbohydrate-active enzymes.
Project description:Transcriptomes of Drosophila melanogaster eye-antennal imaginal discs at three sequential larval stages: late 2nd instar (72h after egg laying (AEL)), mid 3rd instar (96h AEL) and late 3rd instar (120h AEL).
