Piscirickettsia salmonis LF-89 = ATCC VR-1361
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA173790 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Piscirickettsia salmonis LF-89 = ATCC VR-1361 Genome sequencing
| PRJNA229075 | ENA
Project description:Piscirickettsia salmonis LF-89 = ATCC VR-1361 isolate:Lf-89 Raw sequence reads
| PRJNA383157 | ENA
Project description:Piscirickettsia salmonis LF-89 = ATCC VR-1361 Transcriptome or Gene expression
| PRJNA744511 | ENA
Project description:Piscirickettsia salmonis LF-89 = ATCC VR-1361 Transcriptome or Gene expression
| PRJNA413076 | ENA
Project description:Piscirickettsia salmonis LF-89 = ATCC VR-1361 Genome sequencing and assembly
| PRJNA196437 | ENA
Project description:Piscirickettsia salmonis LF-89 = ATCC VR-1361 Genome sequencing and assembly
| PRJNA174046 | ENA
Project description:Piscirickettsia salmonis, the biological agent of SRS (Salmon Rickettsial Syndrome), is a facultative intracellular bacterium that can be divided into two genogroups (LF-89 and EM-90) with different virulence levels and patterns. There are studies that have found co-infection of these genogroups in salmonid farms in Chile, but it is essential to assess whether this competitive interaction within the host is related to virulence and changes in pathogen dynamics. In this work, we studied one isolate from each genogroup, EM-90 and LF-89 . The aim was to evaluate how co-cultures could affect their growth performance and virulence factors expression at in vivo cultures in Atlantic salmon. During in vivo co-cultures, transcriptomic analysis revealed an upregulation of transposases, flagellum-related genes (fliI and flgK), transporters and permeases that could unveil novel virulence effectors used in the early infection process of P. salmonis. Thus, our work has shown that the cohabitation of the genogroups of P. salmonis can modulate their behavior and virulence effectors expression. These data can contribute to new strategies and approaches to improve current health treatments against this salmonid bacterium.
2023-10-03 | GSE235725 | GEO
Project description:Fuentealb2016 - Genome-scale metabolic
reconstruction (iPF215) of Piscirickettsia salmonis LF-89
This model is described in the article:
Genome-scale metabolic
reconstruction for the insidious bacterium in aquaculture
Piscirickettsia salmonis.
Fuentealba P, Aros C, Latorre Y,
Martínez I, Marshall S, Ferrer P, Albiol J, Altamirano
C.
Bioresour. Technol. 2017 Jan; 223:
105-114
Abstract:
Piscirickettsia salmonis is a fish bacterium that causes the
disease piscirickettsiosis in salmonids. This pathology is
partially controlled by vaccines. The lack of knowledge has
hindered its culture on laboratory and industrial scale. The
study describes the metabolic phenotype of P. salmonis in
culture. This study presents the first genome-scale model
(iPF215) of the LF-89 strain of P. salmonis, describing the
central metabolic pathway, biosynthesis and molecule
degradation and transport mechanisms. The model was adjusted
with experiment data, allowing the identification of the
capacities that were not predicted by the automatic annotation
of the genome sequences. The iPF215 model is comprised of 417
metabolites, 445 reactions and 215 genes, was used to reproduce
the growth of P. salmonis (?max 0.052±0.005h(-1)). The
metabolic reconstruction of the P. salmonis LF-89 strain
obtained in this research provides a baseline that describes
the metabolic capacities of the bacterium and is the basis for
developing improvements to its cultivation for vaccine
formulation.
This model is hosted on
BioModels Database
and identified by:
MODEL1610250000.
To cite BioModels Database, please use:
BioModels Database:
An enhanced, curated and annotated resource for published
quantitative kinetic models.
To the extent possible under law, all copyright and related or
neighbouring rights to this encoded model have been dedicated to
the public domain worldwide. Please refer to
CC0
Public Domain Dedication for more information.
2016-12-06 | MODEL1610250000 | BioModels
Project description:Cohabitation of Piscirickettsia salmonis genogroups (LF-89 and EM-90): Synergistic effect on growth dynamics
| PRJNA987513 | ENA
Project description:The aquaculture industry has confronted severe economic losses due to infectious diseases in the last years. Piscirickettsiosis or Salmonid Rickettsial Septicaemia (SRS) is the bacterial disease caused by Piscirickettsia salmonis. This Gram-negative, non-motile, cellular pathogen has the ability to infect, survive, replicate, and propagate in salmonid monocytes/macrophages generating a systemic infection characterized by the colonization of several organs including kidney, liver, spleen, intestine, brain, ovary and gills. In this study, we attempted to determine whether global gene expression differences can be detected in different genetic groups of Atlantic salmon as a result of Piscirickettsia salmonis infection. Moreover, we sought to characterize the fish transcriptional response in order to reveal the mechanisms that might confer resistance in Atlantic salmon to an infection with Piscirickettsia salmonis. In doing so, after challenging with Piscirickettsia salmonis, we selected the families with the highest (HS) and the lowest (LS) recorded susceptibility for gene expression analysis using 32K cGRASP microarrays. Our results revealed in LS families expression changes are linked to iron depletion, as well as, low contents of iron in kidney cells and low bacterial load, indicated that the iron-withholding strategy of innate immunity is part of the mechanism of resistance against Piscirickettsia salmonis. This information contributes to elucidate the underlying mechanisms of resistance to Piscirickettsia salmonis infection in Atlantic salmon and to identify new candidate genes for selective breeding programmes.
2013-01-04 | GSE43255 | GEO