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Arabidopsis thaliana

ABSTRACT: Reprogramming of DNA methylation in pollen guides epigenetic inheritance via small RNA.

PROVIDER: PRJNA174185 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR548294_1.fastq.gz	Fastqsanger.gz
	SRR548294_2.fastq.gz	Fastqsanger.gz
	SRR548295_1.fastq.gz	Fastqsanger.gz
	SRR548295_2.fastq.gz	Fastqsanger.gz
	SRR548296_1.fastq.gz	Fastqsanger.gz

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Reprogramming of DNA methylation in pollen guides epigenetic inheritance via small RNA.

Project description:Epigenetic inheritance is more widespread in plants than in mammals, in part because mammals erase epigenetic information each generation by germline reprogramming. To assess the extent of germline reprogramming in plants, we sequenced the methylome of three haploid cell types from developing pollen: the sperm cell (SC), the vegetative cell, and their precursor the post-meiotic microspore. Whole genome bisulfite sequencing of FACS-purified sperm cells, vegetative nuclei and microspores

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Reprogramming of DNA methylation in pollen guides epigenetic inheritance via small RNA.

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Pseudouridine guides small RNA transport and inheritance via Exportin-t

Project description:Pseudouridine (Ψ) is an isomer of uridine found in ribosomal, transfer and other structural RNAs as well as in some mRNAs and non-coding RNAs, but is difficult to detect in short RNA sequences. Using modified techniques we found Ψ in microRNAs (miRNAs) and their precursors from mammalian and plant cells, primarily at the 5ʹ terminus of the mature miRNA. Small RNAs targeting transposons in reproductive cells (piRNA in testis and easiRNA in pollen) were highly enriched for Ψ, indicating a potential role in epigenetic inheritance. In pollen, pseudouridylated small RNAs were produced by RNA polymerase IV and were localized to sperm cells, as were miRNAs with terminal Ψ. We show that pseudouridylated easiRNAs from pollen contribute to imprinting and the triploid block (chromosome dosage-dependent epigenetic lethality) via the activity of PAUSED/HEN5, the plant homolog of Exportin-t. Exportin-t is required for nuclear export of pseudouridylated tRNA, and we found that PSD is required for cell-cell transport of small RNA in the germline.

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