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Danio rerio

ABSTRACT: Transcriptomic analyses of Myc-induced zebrafish liver cancer

PROVIDER: PRJNA174800 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR566689.fastq.gz	Fastqsanger.gz
	SRR566690.fastq.gz	Fastqsanger.gz
	SRR566691.fastq.gz	Fastqsanger.gz
	SRR566692.fastq.gz	Fastqsanger.gz
	SRR566693.fastq.gz	Fastqsanger.gz

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Effect of obesity on liver immune response to inflammatory stimuli in a diet-obesity model of zebrafish (Danio rerio)

Project description:We used a Diet-Induced-Obesity approach using the zebrafish (Danio rerio) based on overfeeding to analyze the liver transcriptomic modulation in the disease and to determine how the obesity affects the immune response against an acute inflammatory stimulus as the lipopolysacharide (LPS). Overfed zebrafish were obese and showed signs of steatosis in their liver. Furthermore, the gene modulation profile resembled to that observed in humans. After the inflammatory stimulus, there was a clear differential immune response in normal and overfed fish. In normal fish, the response to LPS reported a typical host defensive reaction comparable to the one occurring in stimulated mammals whereas there was not any significantly modulated gene when comparing the expression of liver from LPS-stimulated with non-stimulated obese zebrafish.

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Transcriptomic analyses of Myc-induced zebrafish liver cancer

Project description:To study the characteristics and mechanisms of Myc-induced zebrafish liver tumor, next-generation sequencing-based SAGE analyses were used to examine the transcriptomes of tumor and control samples. The results indicated that ribosome proteins were overwhelmingly up-regulated in the Myc-induced liver tumors. Cross-species analyses showed that the zebrafish Myc model correlated well with Myc transgenic mouse models for liver cancers. The Myc-induced zebrafish liver tumors also possessed molecular signatures highly similar to human hepatocellular carcinoma (HCC). Thus, our zebrafish model demonstrated the conserved role of Myc in promoting hepatocarcinogenesis in all vertebrate species.

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