Homo sapiens
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA175038 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Differences in miRNA Detection Levels are Technology and Sequence Dependent [Agilent array]
| PRJNA175039 | ENA
Project description:Differences in miRNA Detection Levels are Technology and Sequence Dependent [NGS]
| PRJNA175040 | ENA
Project description:Sickle cell transcriptome was analyzed using whole blood clinical specimens on the Affymetrix Human Exon 1.0 ST arrays and Illumina’s deep sequencing technologies. Data analysis indicated a strong concordance (R=0.64) between exon array and RNA-seq in both gene level and exon level expression of transcripts. The magnitude of fold changes in the expression levels for the differentially expressed genes (p<0.05) was found to be higher in RNA-seq than microarrays. However, the arrays outperformed the sequencing technology in the detection of low abundant transcripts. In addition to examining the expression level changes of transcripts, RNA-seq technology was able to identify sequence variation in the expressed transcripts. We also demonstrate herein the ability of RNA-seq technology to discover novel expression outside of the annotated genes.
2012-07-06 | GSE31757 | GEO
Project description:Objective: Detection and analysis of Mongolian medicine Narenmandula the kernel full of adriamycin nephrosis rats kidney tissue expression of miRNA. Methods: The biological information of the differentially expressed miRNA between the normal group, the model group and the Mongolian medicine Narenmandula group was studied by Affymetrix miRNA chip detection. Results: The biological information of miRNA expression in kidney tissues of rats with doxorubicin nephropathy was analyzed by detection, which laid a foundation for further elucidating the multi-target regulatory role of Mongolian medicine Narenmandula in renal function protection and provided data. The biological information of miRNA expression in renal tissues of doxorubicin rats was detected and analyzed using Affymetrix miRNA chip detection technology, which laid a foundation for further elucidating the multi-target regulation effect of the renal function protection and provided data of Mongolian medicine Narenmandula .
2019-12-14 | GSE123776 | GEO
Project description:We have sequenced miRNA libraries from human embryonic, neural and foetal mesenchymal stem cells. We report that the majority of miRNA genes encode mature isomers that vary in size by one or more bases at the 3’ and/or 5’ end of the miRNA. Northern blotting for individual miRNAs showed that the proportions of isomiRs expressed by a single miRNA gene often differ between cell and tissue types. IsomiRs were readily co-immunoprecipitated with Argonaute proteins in vivo and were active in luciferase assays, indicating that they are functional. Bioinformatics analysis predicts substantial differences in targeting between miRNAs with minor 5’ differences and in support of this we report that a 5’ isomiR-9-1 gained the ability to inhibit the expression of DNMT3B and NCAM2 but lost the ability to inhibit CDH1 in vitro. This result was confirmed by the use of isomiR-specific sponges. Our analysis of the miRGator database indicates that a small percentage of human miRNA genes express isomiRs as the dominant transcript in certain cell types and analysis of miRBase shows that 5’ isomiRs have replaced canonical miRNAs many times during evolution. This strongly indicates that isomiRs are of functional importance and have contributed to the evolution of miRNA genes Sequence library of miRNAs from a single sample of human foetal mesenchymal stem cells. Results tested and confirmed by northern blotting. Please note that only raw data files are available for the embryonic and neual samples and thus, directly submitted to SRA (SRX547311, SRX548700, respectively under SRP042115/PRJNA247767)
2014-08-06 | E-GEOD-58734 | biostudies-arrayexpress
Project description:Sickle cell transcriptome was analyzed using whole blood clinical specimens on the Affymetrix Human Exon 1.0 ST arrays and Illumina’s deep sequencing technologies. Data analysis indicated a strong concordance (R=0.64) between exon array and RNA-seq in both gene level and exon level expression of transcripts. The magnitude of fold changes in the expression levels for the differentially expressed genes (p<0.05) was found to be higher in RNA-seq than microarrays. However, the arrays outperformed the sequencing technology in the detection of low abundant transcripts. In addition to examining the expression level changes of transcripts, RNA-seq technology was able to identify sequence variation in the expressed transcripts. We also demonstrate herein the ability of RNA-seq technology to discover novel expression outside of the annotated genes. This Series contains only the Exon array data. 10 patients and 10 healthy subjects participated in this study. Gene chip experiments were carreid out on 6 patients and 4 healthy controls Human Exon 1.0 ST Arrays
2012-07-05 | E-GEOD-31757 | biostudies-arrayexpress
Project description:Explaining Differences in Saturation Levels for Affymetrix GeneChip Arrays
| PRJNA98415 | ENA
Project description:We have investigated the effect of exposure to 150 mg/kg benzo(a)pyrene (BaP) for 3 days on mRNA and miRNA expression levels in adult mouse liver. We used Agilent miRNA array platforms to assess effects of BaP exposure on miRNA expression levels. Our results indicate a distinct lack of effect of BaP of miRNA expression, despite widespread changes in mRNA levels. The data in the attached array files were used a positive control for the Agilent platform, to indicate that the platform was able to detect significant differences in abundance of miRNA between two samples with great differences in miRNA content. Keywords: Toxicology, miRNA
2010-10-28 | GSE19438 | GEO