Project description:A rice transcription factor OsMYC2 plays important role in the regulation of defense responses in rice. To clarify the OsMYC2-responsive genes, we performed a microarray analysis using the Agilent Rice Oligo Microarray (44k, Agilent Technologies, Redwood City, CA, USA). As a result, many defense-related genes including pathogenesis-related (PR) genes were upregulated in the OsMYC2-overexpressing transgenic rice.
Project description:Elevated CO2 (eCO2) condition has influence on developing leaf growth of rice (Oryza sativa cv. Nipponbare), specifically lower than P4 (plastochron number) stage, resulting in decrease in leaf size compared with that grown at ambient CO2 (aCO2). In this case, decrease in leaf size seems to be one of acclimation process at CO2 replete environment despite of the fact that number of tiller increase during CO2 replete periods; however, it is not yet elucidated which endogenous signal play a precise role in depression of developing leaf growth in those process. In this context, to elucidate precise signal interaction between mature and developing leaf of rice at eCO2 environment, we profiled gene expressions of developing rice leaf (P4) using oligo DNA microarray 4X44K RAP-DB (Agilent Technologies, Santa clara, CA).
Project description:Elevated CO2 (eCO2) condition has influence on developing leaf growth of rice (Oryza sativa cv. Nipponbare), specifically lower than P4 (plastochron number) stage, resulting in decrease in leaf size compared with that grown at ambient CO2 (aCO2). In this case, decrease in leaf size seems to be one of acclimation process at CO2 replete environment despite of the fact that number of tiller increase during CO2 replete periods; however, it is not yet elucidated which endogenous signal play a precise role in depression of developing leaf growth in those process. In this context, to elucidate precise signal interaction between mature and developing leaf of rice at eCO2 environment, we profiled gene expressions of mature rice leaf blade (P6) using oligo DNA microarray 4X44K RAP-DB (Agilent Technologies, Santa clara, CA).
Project description:The plant volatilebeta-cyclocitral plays important roles in the regulation of defense responses in rice. To clarify the response to beta-cyclocitral in rice at gene expression level, we performed a microarray analysis using the Agilent Rice Oligo Microarray (44k, custom-made; Agilent Technologies, Redwood City, CA, USA). As a result, treatment of linalool caused high upregulation of many defense-related genes including pathogenesis-related (PR) genes in rice.
Project description:The plant volatile linalool plays important roles in the regulation of defense responses in rice. To clarify the response to linalool in rice at gene expression level, we performed a microarray analysis using the Agilent Rice Oligo Microarray (44k, custom-made; Agilent Technologies, Redwood City, CA, USA). As a result, treatment of linalool caused high upregulation of many defense-related genes including pathogenesis-related (PR) genes in rice.
Project description:The plant volatile linalool plays important roles in the regulation of defense responses in rice. To clarify the response to linalool in rice at gene expression level, we performed a microarray analysis using the Agilent Rice Oligo Microarray (44k, custom-made; Agilent Technologies, Redwood City, CA, USA). As a result, many defense-related genes including pathogenesis-related (PR) genes were upregulated in the linalool synthase-overexpressing transgenic rice.
Project description:The plant hormone jasmonate (JA) plays important roles in the regulation of defense responses in many plants. To clarify the response to JA in rice at gene expression level, we performed a microarray analysis using the Agilent Rice Oligo Microarray (44k, custom-made; Agilent Technologies, Redwood City, CA, USA). As a result, treatment of JA caused high upregulation of many defense-related genes including pathogenesis-related (PR) genes in rice. However, many of these defense-related genes were not upregulated in JA-insensitive transgenic rice plant overexpressing JAZ8deltaC.
Project description:Rare sugars are monosaccharides that are rarely present in nature. One of the rare sugars, D-psicose, had a negative effect on shoot growth of rice. This negative effect to rice growth was observed previously with D-allose, but not observed in other tested rare sugars. To clarify the response to D-psicose in rice at the gene expression level, we performed a microarray analysis using the Agilent Rice Oligo Microarray (44k, custom-made; Agilent Technologies, Redwood City, CA, USA). As a result, treatment of D-psicose caused high induction of many defense-related genes including pathogenesis-related (PR) genes in rice.
Project description:Rare sugars are monosaccharides that are rarely present in nature. One of the rare sugars, D-allose had a negative effect on shoot growth of rice. This negative effect to rice growth was not observed in other tested rare sugars. To clarify the response to the D-allose in rice at gene expression level, we performed a microarray analysis using the Agilent Rice Oligo Microarray (44k, custom-made; Agilent Technologies, Redwood City, CA, USA). As a result, treatment of D-allose caused high induction of many defense-related genes including pathogenesis-related (PR) genes in rice.
Project description:Rare sugars are monosaccharides that are rarely present in nature. To reveal a effect of D-tagatose in plant and the responses to the D-Tagatose in rice at gene expression level, we performed a microarray analysis using the Agilent Rice Oligo Microarray (44K, custom-made; Agilent Technologies, Redwood City, CA, USA). As a result, there are no significant differences on the gene expression patterns between in mock- and D-tagatose-treated rice plants, and several PR-protein genes which had been reported to be induced by D-allulose or D-allose treatment, were compared by that with D-tagatose treatment in rice.