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ABSTRACT: Genome-wide analysis of regulation of gene expression and H3K9me2 distribution by JIL-1 kinase mediated histone H3S10 phosphorylation in drosophila

PROVIDER: PRJNA175626 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1145610.fastq.gz	Fastqsanger.gz
	SRR1145620_1.fastq.gz	Fastqsanger.gz
	SRR1145620_2.fastq.gz	Fastqsanger.gz
	SRR1145611.fastq.gz	Fastqsanger.gz
	SRR1145621_1.fastq.gz	Fastqsanger.gz

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JASPer controls interphase histone H3S10 phosphorylation by chromosomal kinase JIL-1 in Drosophila

Project description:In Drosophila the chromosomal kinase JIL-1 is responsible for most interphase histone H3S10 phosphorylation and has been proposed to protect active chromatin from acquiring heterochromatic marks, like dimethylated histone H3K9 (H3K9me2) and HP1. Here, we show that JIL-1’s targeting to chromatin depends on a PWWP domain-containing protein JASPer (JIL-1 Anchoring and Stabilizing Protein). The JASPer-JIL-1 (JJ)-complex is the major form of the kinase in vivo and is targeted to active genes and telomeric transposons via binding of the PWWP domain of JASPer to H3K36me3 nucleosomes, where the complex modulates the transcriptional output. JIL-1 and JJ-complex depletion in cycling cells lead to small changes in H3K9me2 distribution at active genes and telomeric transposons. Finally, we identify several interactors of the endogenous JJ-complex and propose that JIL-1 not only prevents heterochromatin formation but also coordinates chromatin-based regulation in the transcribed part of the genome.

2019-10-09 | PXD012790 | Pride

Genome-wide analysis of H3K9me2 and JIL-1 kinase mediated histone H3S10 phosphorylation in drosophila [ChIP-seq: 13 samples]

Project description:In this study we have determined the genome-wide relationship of JIL-1 kinase mediated H3S10 phosphorylation with gene expression and the distribution of the epigenetic H3K9me2 mark. We show in wild-type salivary gland cells that the H3S10ph mark is predominantly enriched at active genes whereas the H3K9me2 mark largely is associated with inactive genes.

2014-02-12 | GSE54438 | GEO

JASPer controls interphase H3S10 phosphorylation by chromosomal kinase JIL-1 in Drosophila (Spike-in ChIP-seq experiments)

Project description:In flies, the chromosomal kinase JIL-1 is responsible for most interphase H3S10 phosphorylation and has been proposed to protect active chromatin from acquiring heterochromatic marks like H3K9me2 and HP1. Here, we show that JIL-1’s targeting to chromatin depends on a new PWWP domain containing protein JASPer (JIL-1 Anchoring and Stabilizing Protein). The JASPer/JIL-1 (JJ)-complex is the major form of the kinase in vivo and is targeted to active genes and telomeric transposons via binding of the PWWP domain of JASPer to H3K36me3 nucleosomes. Put in place, the complex modulates the transcriptional output. JIL-1 and JJ-complex depletion in cycling cells induce small changes in H3K9me2 distribution at active genes and telomeric transposons. Finally, we identified many new interactors of the endogenous JJ-complex and propose that JIL-1 not only prevents heterochromatinisation, but also coordinates chromatin based regulation in the transcribed part of the genome.

2019-09-19 | GSE130112 | GEO

JASPer controls interphase H3S10 phosphorylation by chromosomal kinase JIL-1 in Drosophila (RNA-seq experiments)

2019-09-19 | GSE128454 | GEO

JASPer controls interphase H3S10 phosphorylation by chromosomal kinase JIL-1 in Drosophila (Sonication ChIP-seq experiments)

2019-09-19 | GSE128456 | GEO

JASPer controls interphase H3S10 phosphorylation by chromosomal kinase JIL-1 in Drosophila (MNase ChIP-seq experiments)

2019-09-19 | GSE128455 | GEO

Drosophila melanogaster

Project description:Genome-wide analysis of H3K9me2 and JIL-1 kinase mediated histone H3S10 phosphorylation in drosophila [ChIP-seq: 13 samples]

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JASPer controls interphase H3S10 phosphorylation by chromosomal kinase JIL-1 in Drosophila

Project description:JASPer controls interphase H3S10 phosphorylation by chromosomal kinase JIL-1 in Drosophila

| PRJNA527788 | ENA

JASPer controls interphase H3S10 phosphorylation by chromosomal kinase JIL-1 in Drosophila (RNA-seq experiments)

Project description:JASPer controls interphase H3S10 phosphorylation by chromosomal kinase JIL-1 in Drosophila (RNA-seq experiments)

| PRJNA527792 | ENA

H3S10ph broadly marks early-replicating domains in interphase ESCs and shows reciprocal antagonism with H3K9me2

Project description:Pervasive phosphorylation of histone H3 at serine 10 (H3S10ph) by Aurora B/C plays an important role in mitosis; however, this mark has also been observed at specific genic promoters and enhancers in interphase, implicating mitosis-independent functions. Using the FUCCI cell cycle reporter, we found that 30% of the genome is persistently marked with H3S10ph in interphase mouse embryonic stem cells (ESCs). H3S10ph demarcates broad gene-rich euchromatic regions in G1 and shows remarkable correlation with domains of early DNA replication timing (RT). Consistent with mitosis-independent H3S10 kinase activity, this pattern was preserved in ESCs treated with hesperidin, a potent inhibitor of Aurora B/C. Disruption of H3S10ph by expression of non-phosphorylatable H3.3S10A results in ectopic spreading of H3K9me2 into adjacent H3S10ph-enriched euchromatic regions, mimicking the phenotype observed in Drosophila JIL-1 kinase mutants. Conversely, H3S10ph domains expand in interphase Glp-/- ESCs, revealing that H3S10ph expansion is restricted by H3K9me2. Strikingly, spreading of H3S10ph at RT transition regions (TTRs) is accompanied by aberrant strand-biased transcription initiation of genes and repetitive elements co-oriented with the replication fork, indicating that H3K9me2 plays a critical role in establishing repressive chromatin on the leading strand. Finally, we show that H3S10ph is also present in interphase murine embryonic fibroblasts (MEFs), but is restricted to intragenic regions of actively transcribing genes. Our study provides a detailed map of interphase H3S10ph in ESCs and MEFs, uncovering a previously unappreciated crosstalk between the opposing marks H3S10ph and H3K9me2, and a role for the latter in ensuring appropriate transcription at TTRs in ESCs.

2017-11-20 | GSE97947 | GEO

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