Project description:Tryblidiopsis pinastri (Leotiomycetes, Rhytismatales) was described from Picea abies in Europe and was also thought to occur on North American Picea. However, previously published sequences of Picea foliar endophytes from Eastern Canada suggested the presence of at least two cryptic Tryblidiopsis species, distinct from T. pinastri and other known species. Our subsequent sampling of Tryblidiopsis ascomata from dead attached Picea glauca branches resulted in the collection of a putatively undescribed species previously isolated as a P. glauca endophyte. Morphological evidence combined with phylogenetic analyses based on nuclear ribosomal internal transcribed spacer (ITS) and large subunit ribosomal (LSU) DNA sequences support the distinctiveness of this species, described here as T. magnesii.

| S-EPMC7241680 | biostudies-literature

Organellar Genomes of White Spruce (Picea glauca): Assembly and Annotation.

Project description:The genome sequences of the plastid and mitochondrion of white spruce (Picea glauca) were assembled from whole-genome shotgun sequencing data using ABySS. The sequencing data contained reads from both the nuclear and organellar genomes, and reads of the organellar genomes were abundant in the data as each cell harbors hundreds of mitochondria and plastids. Hence, assembly of the 123-kb plastid and 5.9-Mb mitochondrial genomes were accomplished by analyzing data sets primarily representing low coverage of the nuclear genome. The assembled organellar genomes were annotated for their coding genes, ribosomal RNA, and transfer RNA. Transcript abundances of the mitochondrial genes were quantified in three developmental tissues and five mature tissues using data from RNA-seq experiments. C-to-U RNA editing was observed in the majority of mitochondrial genes, and in four genes, editing events were noted to modify ACG codons to create cryptic AUG start codons. The informatics methodology presented in this study should prove useful to assemble organellar genomes of other plant species using whole-genome shotgun sequencing data.

| S-EPMC4758241 | biostudies-literature

Picea glauca

Project description:Picea glauca WS77111 genome sequencing

| PRJNA242552 | ENA

Picea glauca

Project description:Molecular events of apical bud formation in white spruce

| PRJNA131767 | ENA

Picea glauca

Project description:Direct measurement of heritable gene expression within single individuals from wild populations.

| PRJNA152705 | ENA

Picea glauca

Project description:White spruce bark cell-type specific transcriptomes