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Escherichia coli str. K-12 substr. MG1655star

ABSTRACT: Genome-wide analysis of FNR and σ70 in E. coli under aerobic and anaerobic growth conditions.

PROVIDER: PRJNA176149 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR576933.fastq.gz	Fastqsanger.gz
	SRR576934.fastq.gz	Fastqsanger.gz
	SRR576935.fastq.gz	Fastqsanger.gz
	SRR576936.fastq.gz	Fastqsanger.gz
	SRR576937.fastq.gz	Fastqsanger.gz

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Genome-wide analysis of FNR and σ70 in E. coli under aerobic and anaerobic growth conditions.

Project description:We found many binding sites for FNR under glucose fermentative anaerobic growth conditions. Also, many binding sites were identified for σ70 under both aerobic and anaerobic growthin conditions. Descirbed in the manuscript "Genome-scale Analysis of E. coli FNR Reveals the Complexity of Bacterial Regulon Structure"

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Project description:Mapping the occupancy of FNR, HNS, and IHF throughout the genome of Escherchia coli MG1655 K-12 using an affinity purified antibody under anerobic growth conditions. We also mapped the binding of the M-CM-^_ subunit of RNA Polymerase under both aerobic and anaerobic growth conditions. As a control, we also performed ChIP-chip on FNR in a M-bM-^HM-^Ffnr mutant strain of Escherchia coli MG1655 K-12. We also examined FNR immunoprecipitation at various FNR concentrations using IPTG and Ptac::fnr (PK8263). The M-bM-^HM-^Fhns/M-bM-^HM-^FstpA strains were also used. Descirbed in the manuscript Genome-scale Analysis of E. coli FNR Reveals the Complexity of Bacterial Regulon Structure Mapping of occupancy of FNR, NNS, IHF and M-CM-^_ of RNAP in the genome of Escherchia coli MG1655 K-12 under aerobic or anaerobic growth conditions. Immunoprecipitated DNA compared to INPUT for each sample.

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Project description:Mapping the occupancy of FNR, HNS, and IHF throughout the genome of Escherchia coli MG1655 K-12 using an affinity purified antibody under anerobic growth conditions. We also mapped the binding of the ß subunit of RNA Polymerase under both aerobic and anaerobic growth conditions. As a control, we also performed ChIP-chip on FNR in a ∆fnr mutant strain of Escherchia coli MG1655 K-12. We also examined FNR immunoprecipitation at various FNR concentrations using IPTG and Ptac::fnr (PK8263). The ∆hns/∆stpA strains were also used. Descirbed in the manuscript Genome-scale Analysis of E. coli FNR Reveals the Complexity of Bacterial Regulon Structure

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Genome-wide analysis of FNR and M-OM-^C70 in E. coli under aerobic and anaerobic growth conditions.

Project description:We found many binding sites for FNR under glucose fermentative anaerobic growth conditions. Also, many binding sites were identified for M-OM-^C70 under both aerobic and anaerobic growthin conditions. Descirbed in the manuscript "Genome-scale Analysis of E. coli FNR Reveals the Complexity of Bacterial Regulon Structure" Examination of occupancy of FNR adn M-OM-^C70 under aerobic and anaerobic growth in conditions.

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Expression analysis of Escherichia coli MG1655 K-12 WT and M-bM-^HM-^Ffnr mutant

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Escherichia coli str. K-12 substr. MG1655

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RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655

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