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Schizosaccharomyces pombe

ABSTRACT: Clustered regulatory signals at nucleosome-depleted regions punctuate a constant nucleosomal landscape in Schizosaccharomyces pombe [NGS_Illumina]

PROVIDER: PRJNA178153 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR605665.fastq.gz	Fastqsanger.gz
	SRR605666.fastq.gz	Fastqsanger.gz
	SRR605667.fastq.gz	Fastqsanger.gz
	SRR605668.fastq.gz	Fastqsanger.gz
	SRR605669.fastq.gz	Fastqsanger.gz

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Project description:Clustered regulatory signals at nucleosome-depleted regions punctuate a constant nucleosomal landscape in Schizosaccharomyces pombe [Affymetrix]

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S. pombe genome-wide nucleosome mapping reveals positioning mechanisms distinct from S. cerevisiae

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Project description:We have used micrococcal nuclease (MNase) digestion followed by deep sequencing in order to obtain a higher-resolution map than previously available of nucleosome positions in the fission yeast, Schizosaccharomyces pombe. Our data confirm an unusually short average nucleosome repeat length, ~152 bp, in fission yeast and that transcriptional start sites (TSSs) are associated with nucleosome-depleted regions (NDRs), ordered nucleosome arrays downstream and less regularly spaced upstream nucleosomes. In addition, we found enrichments for associated function in four of eight groups of genes clustered according to chromatin configurations near TSSs. At replication origins, our data revealed asymmetric localization of Pre-Replication Complex (pre-RC) proteins within large NDRsM-bM-^@M-^Ta feature that is conserved in fission and budding yeast and is therefore likely to be conserved in other eukaryotic organisms. Examination of nucleosome positioning in Schizosaccharomyces pombe

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