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Clostridium pasteurianum DSM 525 = ATCC 6013

ABSTRACT: Clostridium pasteurianum DSM 525 = ATCC 6013 Genome sequencing and assembly

PROVIDER: PRJNA182067 | ENA |

REPOSITORIES: ENA

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Dataset's files

Source:

			Action	DRS
	ANZB01.dat.gz	Other
	ANZB01.fasta.gz	Fasta.gz
	ANZB01.master.dat	Other
	ANZB01.dat.gz	Other

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Clostridium pasteurianum DSM 525 = ATCC 6013

Project description:Clostridium pasteurianum DSM 525 = ATCC 6013 Genome sequencing

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Clostridium pasteurianum DSM 525 = ATCC 6013

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Clostridium pasteurianum DSM 525 = ATCC 6013

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Draft Genome Sequence of Type Strain Clostridium pasteurianum DSM 525 (ATCC 6013), a Promising Producer of Chemicals and Fuels.

Project description:Clostridium pasteurianum, an anaerobic bacterium able to utilize atmospheric free nitrogen for biosynthesis, has recently been proven to be a promising producer of chemicals and fuels, such as 1,3-propanediol and n-butanol. Here, we report the high-quality draft genome sequence of DSM 525, a type strain of C. pasteurianum.

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Complete Genome Sequence of the Nitrogen-Fixing and Solvent-Producing Clostridium pasteurianum DSM 525.

Project description:Here, we report on the closed genome sequence of Clostridium pasteurianum DSM 525, which is an anaerobic, Gram-positive and endospore-forming organism. C. pasteurianum can fix N2 and produce solvents such as butanol and 1,3-propanediol from carbohydrates. The genome consists of a single 4,350,673-bp replicon.

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Clostridium pasteurianum strain:W5

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Clostridium pasteurianum TK strain:TK

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Transcriptome analysis of the opportunist pathogenic bacterium Clostridium perfringens ATCC 13124 grown in the presence of host polysaccharide hyaluronic acid and mucin

Project description:Purpose: The purpose of this study is to clarify the response of Clostridium perfringens ATCC 13124 to host polysaccharide. Methods: Clostridium perfringens ATCC 13124 cells were cultured anaerobically in a medium containing Minimal medium-like condition Poor + medium, medium in which hyaluronic acid or mucin was added to Poor + medium. Total RNA was extracted from bacterial cells by the Hot-Phenol method. Samples for RNA-seq were prepared according to the Illmina protocol available from the manufacturer. Array leads passed through quality filters were analyzed at the transcript isoform level using bowtie v 1.1.2. Results: Using the optimized data analysis workflow, we mapped about 50 million sequence leads per sample to the whole genome of Clostridium perfringens ATCC 13124. In addition, 2735 transcripts in C. perfringens ATCC 13124 were identified using a Bowtie aligner. Lead counts per genome were extracted from known gene annotations using the HTSeq program.

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Closed Genome Sequence of Clostridium pasteurianum ATCC 6013.

Project description:We report here the closed genome of Clostridium pasteurianum ATCC 6013, a saccharolytic, nitrogen-fixing, and spore-forming Gram-positive obligate anaerobe. The organism is of biotechnological interest due to the production of solvents (butanol and 1,3-propanediol) but can be associated with food spoilage. The genome comprises a total of 4,351,223 bp.

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