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Freshwater metagenome strain:from synthetic mineral medium in Toronto, ON

ABSTRACT: Enriched contaminated groundwater dechlorinating community (KB-1) from synthetic mineral medium in Toronto, ON

PROVIDER: PRJNA185209 | ENA |

REPOSITORIES: ENA

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groundwater metagenome

Project description:Groundwater dechlorinating microbial communities from synthetic mineral medium in Toronto, Ontario, Canada - Site contaminated with chlorinated ethenes metagenome

| PRJNA337103 | ENA

Gene expression in synthetic nasal medium

2013-04-10 | GSE43712 | GEO

Gene expression in synthetic nasal medium

Project description:Staphylococcus aureus prefers the human anterior nares as its habitat, but nothing is known about the nutritional situation in this ecological niche. Analysis of nasal secretions showed a complex mixture of nutrients at low concentration. Based on these findings a synthetic nasal medium (SNM) was composed, mimicking nasal secretions. We used microarrays in order to investigate pathways and expression patterns important in a synthetic medium mimicking nasal secretions compared to standard laboratory complex medium Staphylococcus aureus USA300 was inoculated in complex medium (BM) or synthetic nasal medium (SNM) to an OD578nm of 0.005 and grown under aerobic conditions until OD578nm of 0.02. RNA was stabilized and extracted at this early growth phase and hybridization was made on Affymetrix microarrays. The aim was to identify genes which are important during growth under the limited conditions present during colonization of human nares

2013-04-10 | E-GEOD-43712 | biostudies-arrayexpress

Comparative expression analysis between logarithmic and stationary phase of T. thermophilus HB8 on a synthetic medium

Project description:We observed the expression profile of the total mRNA of Thermus thermophilus HB8 wild-type strain grown in a synthetic medium at 70°C.

2008-08-03 | GSE11671 | GEO

mineral

Project description:Mineral types and ectomycorrhizal fungal species select the bacterial community structures in mineral-amended mesh bags

| PRJNA495753 | ENA

Time course of the mRNA expression in wild-type Thermus thermophilus HB8 strain grown on a synthetic medium

Project description:We observed the expression profile of the total mRNA of wild-type Thermus thermophilus HB8 strain grown in a synthetic medium at 70°C. Keywords: time course

2010-10-15 | GSE19747 | GEO

Characterization of four TCE-dechlorinating microbial enrichments grown with different cobalamin stress and methanogenic conditions

Project description:Four stable and robust TCE-dechlorinating microbial communities were enriched from TCE-contaminated groundwater under four different conditions exploring two parameters, high and low methanogenic activity (Meth and NoMeth), with and without vitamin B12 supplement (MethB12 and NoMethB12, Meth and NoMeth, respectively). Identical amounts of lactate (2.7 mmol) and TCE (20 μl) were supplied as electron donor and electron acceptor. All four cultures were capable of reductively dechlorinating TCE to VC and ethene. Genomic DNA of the four enrichments was applied on a quad-Dhc-genome microarray in order to characterize the gene content of Dehalococcoides species present in the four enrichments

2013-06-30 | GSE33530 | GEO

Comparative expression analysis between logarithmic and stationary phase of T. thermophilus HB8 on a synthetic medium

Project description:We observed the expression profile of the total mRNA of Thermus thermophilus HB8 wild-type strain grown in a synthetic medium at 70°C. Three wild-type T. thermophilus HB8 strains were pre-cultured at 70°C for 16 h in 3 ml of synthetic medium. The cells (2 ml) were inoculated into 500 ml of the same medium and then cultivated at 70°C. Cells were collected after 10 h and 20 h, and then crude RNA was extracted. The expression of each mRNA at each time point was analyzed on a GeneChip as described under Sample Description Sheet of each sample in this website.

2008-08-03 | E-GEOD-11671 | biostudies-arrayexpress

A selection of Saccharomyces cerevisiae promoters for biotechnological applications aiming at the use of synthetic glycerol medium

Project description:Glycerol offers several advantages as a substrate for biotechnological applications. An important step towards using the popular production host Saccharomyces cerevisiae for glycerol-based bioprocesses have been recent studies in which commonly used S. cerevisiae strains were engineered to grow in synthetic medium containing glycerol as the sole carbon source. In order to boost extensive S. cerevisiae metabolic engineering incentives aiming at the use of glycerol, we realized that promoters with predictable expression levels in synthetic glycerol medium were required. In the current study, we used transcriptome analysis and a yECitrine-based fluorescence reporter assay to select and characterize useful 25 promoters for driving expression of target genes in S. cerevisiae under the given conditions. The promoters of the genes ALD4 and ADH2 showed 4.2- and 3-fold higher activities compared to the well-known strong TEF1 promoter. Moreover, the collection contains promoters with graded activities in synthetic glycerol medium and different degrees of glucose repression. To demonstrate the general applicability of the promoter collection, we successfully used a subset of the characterized promoters with graded activities in order to optimize growth on glycerol in an engineered derivative of CEN.PK, in which glycerol catabolism exclusively occurs via a non-native DHA pathway.

2018-03-31 | GSE109036 | GEO

Time course of the mRNA expression in wild-type Thermus thermophilus HB8 strain grown on a synthetic medium

Project description:We observed the expression profile of the total mRNA of wild-type Thermus thermophilus HB8 strain grown in a synthetic medium at 70Â°C. Keywords: time course Wild-type T. thermophilus HB8 strains were pre-cultured at 70Â°C for 16 h in 3 ml of synthetic medium. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70Â°C. Cells were collected after 360, 480, 600, 720, 840, 960, 1080, 1200, 1320 and 1440 min, and then crude RNA was extracted. The expression of each mRNA at each time point was analyzed on a GeneChip as described under 'Description' field of each Sample.

2010-10-15 | E-GEOD-19747 | biostudies-arrayexpress

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