Project description:We exposed Salmonella TA100 cells to 3 concentrations of MX that produced a linear concentration-response for mutagenesis with little cell killing. We measured mutagenesis, survival, and global gene expression. We used custom-spotted glass slides as our microarray platform and identified genes whose expressions were altered by MX treatment using three methods: (1) a Bayesian t-test, (2) an operon analysis that assumes if one gene in an operon is differentially expressed then all genes in that operon are differentially expressed, and (3) a monotonic-expression response to increasing doses of MX. The resulting list of genes was analyzed for functional and KEGG pathway representation. Keywords: dose response

2007-02-14 | GSE7034 | GEO

Paratrimastix pyriformis ATCC50562

Project description:This protist is a free-living nanoflagellate

| PRJNA12673 | ENA

Paratrimastix pyriformis ATCC50562

Project description:An EST project has begun for this protist

| PRJNA12674 | ENA

Geosiphon pyriformis strain:uo1

Project description:Geosiphon pyriformis strain:uo1 Genome sequencing and assembly

| PRJNA610605 | ENA

The free-living flagellate Paratrimastix pyriformis uses a distinct mitochondrial carrier to balance adenine nucleotide pools.

Project description:Paratrimastix pyriformis is a free-living flagellate thriving in low-oxygen freshwater sediments. It belongs to the group Metamonada along with human parasites, such as Giardia and Trichomonas. Like other metamonads, P. pyriformis has a mitochondrion-related organelle (MRO) which in this protist is primarily involved in one-carbon folate metabolism. The MRO contains four members of the solute carrier family 25 (SLC25) responsible for the exchange of metabolites across the mitochondrial inner membrane. Here, we characterise the function of the adenine nucleotide carrier PpMC1 by thermostability shift and transport assays. We show that it transports ATP, ADP and, to a lesser extent, AMP, but not phosphate. The carrier is distinct in function and origin from both ADP/ATP carriers and ATP-Mg/phosphate carriers, and it most likely represents a distinct class of adenine nucleotide carriers.

| S-EPMC10251735 | biostudies-literature

Pseudomonas putida strain:MX-2

Project description:Pseudomonas putida strain:MX-2 Genome sequencing

| PRJNA594810 | ENA

Candidatus Methanocrinis natronophilus strain:Mx

Project description:Candidatus Methanocrinis natronophilus strain:Mx Genome sequencing and assembly

| PRJNA941178 | ENA