Arabidopsis thaliana
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA186928 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:rs11-09_meotic - time course expression profile of ap1-cal after induction of an ap1-transgene - CDKs are major regulators of the mitotic as well as the meiotic cell cycle. In comparison with the mitotic cell cycle much less is known about the regulation of meiosis, especially in plants. One of the reasons for this is the very low abundance and the difficult accessibility of cell undergoing meiosis. We have developed a system to enrich for meiocytes. This will be the material with which we will search for CDK substrates in a biochemical approach. To first get an overview about the meiotic genes expressed in this system, we want to perform here a time series at four time points after the induction that leads to the synchronized development of meiocytes. The in the microarray identified genes, will then set the frame for the upcoming biochemical experiments. - An ap1-cal double mutants carrying an Glucocorticoid-receptor fusion to AP1 was indcued and samples were collected 2 days after induction (dai), 8dapi, 9dai, 10dai and 11dai. Then, the expression profile of 2dai were compared with 8dai, 8 with 9, 9 with 10, and 10 with 11. 12 dye-swap - time course
2013-02-01 | E-GEOD-43646 | biostudies-arrayexpress
Project description:rs11-09_meotic - time course expression profile of ap1-cal after induction of an ap1-transgene - CDKs are major regulators of the mitotic as well as the meiotic cell cycle. In comparison with the mitotic cell cycle much less is known about the regulation of meiosis, especially in plants. One of the reasons for this is the very low abundance and the difficult accessibility of cell undergoing meiosis. We have developed a system to enrich for meiocytes. This will be the material with which we will search for CDK substrates in a biochemical approach. To first get an overview about the meiotic genes expressed in this system, we want to perform here a time series at four time points after the induction that leads to the synchronized development of meiocytes. The in the microarray identified genes, will then set the frame for the upcoming biochemical experiments. - An ap1-cal double mutants carrying an Glucocorticoid-receptor fusion to AP1 was indcued and samples were collected 2 days after induction (dai), 8dapi, 9dai, 10dai and 11dai. Then, the expression profile of 2dai were compared with 8dai, 8 with 9, 9 with 10, and 10 with 11.
2013-02-01 | GSE43646 | GEO
Project description:This experiment describes gene expression during early Arabidopsis flower development. We used a 35S:AP1-GR ap1 cal line to induce synchronized flower development by specifically activating the AP1-GR fusion protein in ap1 cal inflorescence-like meristems through dexamethasone treatment. Tissue samples were collected immediately after the treatment, as well as at one-day intervals for the following five days. The expression profiles of the individual samples were then analyzed by gene expression profiling using whole-genome microarrays (Operon). Keywords: time course
2006-04-10 | GSE4594 | GEO
Project description:This experiment describes gene expression after the activation of APETALA1-GR, to study and identify AP1 target genes. We used a 35S:AP1-GR ap1 cal line to induce a synchronized response activating the AP1-GR fusion protein in ap1 cal inflorescence-like meristems through dexamethasone treatment. Tissue samples were collected immediately after the treatment, as well as subsequent timepoints. The expression profiles of the individual samples were then analyzed by gene expression profiling using whole-genome oligonucleotide arrays (non-commercial; Meyerowitz Lab Arabidopsis Operon Array v4). Keywords: time course
2010-04-02 | GSE20183 | GEO
Project description:This experiment describes gene expression after the activation of APETALA1-GR, to study and identify AP1 target genes. We used a 35S:AP1-GR ap1 cal line to induce a synchronized response activating the AP1-GR fusion protein in ap1 cal inflorescence-like meristems through dexamethasone treatment. Tissue samples were collected immediately after the treatment, as well as subsequent timepoints. The expression profiles of the individual samples were then analyzed by gene expression profiling using whole-genome oligonucleotide arrays (non-commercial; Meyerowitz Lab Arabidopsis Operon Array v4). Keywords: time course
2010-04-02 | GSE20182 | GEO
Project description:This experiment describes gene expression after the activation of APETALA1-GR, to study and identify AP1 target genes. We used a 35S:AP1-GR ap1 cal line to induce a synchronized response activating the AP1-GR fusion protein in ap1 cal inflorescence-like meristems through dexamethasone treatment. Tissue samples were collected immediately after the treatment, as well as subsequent timepoints. The expression profiles of the individual samples were then analyzed by gene expression profiling using whole-genome oligonucleotide arrays (Agilent, custom-commercial). Keywords: time course
2010-04-02 | GSE20139 | GEO
Project description:Meiotic time-course expression profile
| PRJNA123487 | ENA
Project description:Plant inflorescence-to-floral phase transition is an important developmental stage, in which floral cell identities and many traits of reproductive organs are determined. Two MADS-domain transcription factors, APETALA1 (AP1) and CAULIFLOWER (CAL), have been known as master regulators controlling the early stage of the phase transition in Arabidopsis. In plants with loss-of-function alleles of ap1 and cal double mutations, flower development is heavily delayed at the flower initiation stage and accumulate a large number of inflorescence-like meristem cells compared to wild-type plants, resulting in a cauliflower-like phenotype. To facilitate investigation on molecular mechanisms during inflorescence-to-floral phase transition, an inducible system of synchronized floral development has been developed, in which ap1,cal inflorescence-like meristem cells express a fusion protein of AP1 and the hormone-binding domain of the rat glucocorticoid receptor (GR) driven by 35S constitutive promoter. When inflorescences of 35S:AP1-GR ap1,cal plants are treated by steroid hormone dexamethasone as the activator to allow the AP1-GR fusion protein translocate into nucleus, inflorescence-to-floral phase transition is triggered and plants start to produce hundreds of relatively synchronized floral buds. To explore molecular basis at early stage of flower development in Arabidopsis, we used the inducible system of synchronized floral development (35S:AP1-GR ap1,cal) to profile transcriptome change of meristem cells during inflorescence-to-floral phase transition by strand-specific RNA-sequencing.
2018-12-01 | GSE59677 | GEO
Project description:This experiment describes gene expression after the activation of APETALA1-GR, to study and identify AP1 target genes. We used a 35S:AP1-GR ap1 cal line to induce a synchronized response activating the AP1-GR fusion protein in ap1 cal inflorescence-like meristems through dexamethasone treatment. Tissue samples were collected immediately after the treatment, as well as subsequent timepoints. The expression profiles of the individual samples were then analyzed by gene expression profiling using whole-genome oligonucleotide arrays (non-commercial; Meyerowitz Lab Arabidopsis Operon Array v4). Keywords: time course Four sets of biologically independent tissue samples were collect at 0, 2, 4 ,8, and 12 hours after the application of dexamethasone (Dex-; activation of the AP1-GR fusion protein) or a mock solution (Mock-; control). In each of the biological replicates of the time course experiments, all the samples derived from dexamethasone (Dex)-treated plants were labeled with one dye (i.e., Cy3), and all the samples derived from the corresponding Mock-treated plants were labeled with the alternative dye (i.e., Cy5). The dyes used for labeling RNA from a given treatment type (Dex and Mock) were switched for two of the replicate experiments, to reduce dye-related artifacts. Dex- and Mock-derived samples for each timepoint and biological replicate were co-hybridized. This experimental setup resulted in a total of 5 hybridizations per set (0h, 2h, 4, 8h, and 12h; Dex vs. Mock at each timepoint), and two biological replicate sets labeled with each dye polarity (Mock-Cy3/Dex-Cy5, and vice versa). The combined ratio data results are available as a supplementary file on the Series record.
2010-04-02 | E-GEOD-20182 | biostudies-arrayexpress
Project description:This experiment describes gene expression after the activation of APETALA1-GR, to study and identify AP1 target genes. We used a 35S:AP1-GR ap1 cal line to induce a synchronized response activating the AP1-GR fusion protein in ap1 cal inflorescence-like meristems through dexamethasone treatment. Tissue samples were collected immediately after the treatment, as well as subsequent timepoints. The expression profiles of the individual samples were then analyzed by gene expression profiling using whole-genome oligonucleotide arrays (non-commercial; Meyerowitz Lab Arabidopsis Operon Array v4). Keywords: time course Four sets of biologically independent tissue samples were collect at 0, 2, 4 ,8, and 12 hours after the application of dexamethasone (Dex-; activation of the AP1-GR fusion protein) or a mock solution (Mock-; control). In each of the biological replicates of the time course experiments, all the samples derived from dexamethasone (Dex)-treated plants were labeled with one dye (i.e., Cy3), and all the samples derived from the corresponding Mock-treated plants were labeled with the alternative dye (i.e., Cy5). The dyes used for labeling RNA from a given treatment type (Dex and Mock) were switched for two of the replicate experiments, to reduce dye-related artifacts. Dex- and Mock-derived samples for each timepoint and biological replicate were co-hybridized. This experimental setup resulted in a total of 5 hybridizations per set (0h, 2h, 4, 8h, and 12h; Dex vs. Mock at each timepoint), and two biological replicate sets labeled with each dye polarity (Mock-Cy3/Dex-Cy5, and vice versa). The combined ratio data results are available as a supplementary file on the Series record.
2010-04-02 | E-GEOD-20183 | biostudies-arrayexpress