Project description:The acarviose metabolite acarbose is an a glucosidase inhibitor produced by Actinoplanes sp. SE50/110. It is medically important because it is used in the treatment of type 2 diabetes. In this work a comprehensive proteome analysis of Actinoplanes sp. SE50/110 was carried out. The associated txt and RAW files were used for two different analyses and publications. While one study focused on a comparative analysis of Actinoplanes sp. SE50/110 to elucidate differences in the proteome cultures that were grown with either maltose or glucose, the other study applied spectral counting and analyzed only the maltose-grown cultures to determine the major proteins and their location in the cell. The txt files for the comparative data are labeled as "heavy_light" and of the spectral counting data as "light". Both datasets were derived from the same RAW files.
Project description:Cultivation in maltose minimal media and sampling at different time point (t2 = 96h; t3=118h; t4 = 142h; t5 = 166h) of Actinoplanes sp. SE50/110 empty vector control and Actinoplanes sp. SE50/110 ACSP50_0507 overxepression mutant. RNAseq material and methods