Project description:time-course salt stress experiment of model legume Medicago truncatula roots using Affymetrix Medicago Array, aimed to dig some useful gene for improve salt resistance for legumes and other crops
Project description:time-course salt stress experiment of model legume Medicago truncatula roots using Affymetrix Medicago Array, aimed to dig some useful gene for improve salt resistance for legumes and other crops
Project description:we used two-dimensional gel electrophoresis and mass spectrometry to characterize the proteome-level changes associated with salt stress response in Medicago sativa cv. Zhongmu-1 and Medicago truncatula cv. Jemalong A17 roots. The tandem mass spectrometry analysis of the differentially accumulated proteins resulted in the identification of 60 and 26 proteins in Zhongmu-1 and Jemalong A17 roots, respectively.
Project description:This SuperSeries is composed of the following subset Series:; GSE13907: Medicago truncatula seedlings grown two weeks in hydroponics media then carry out salt stress; GSE13921: Medicago truncatula germinated seedlings grown in Petri dishes for 3 days then carry out salt stress Experiment Overall Design: Refer to individual Series
Project description:time-course salt stress experiment of model legume Medicago truncatula roots using Affymetrix Medicago Array, aimed to dig some useful gene for improve salt resistance for legumes and other crops Experiment Overall Design: 6 samples of Medicago truncatula seedlings Experiment Overall Design: grown two weeks in hydroponics media Experiment Overall Design: 200mM NaCl salt stress Experiment Overall Design: material collect at 0, 1, 2, 5, 10, 24 hour after salt stress beginning Experiment Overall Design: 2 ug of total RNA was used to synthesize doublestranded cDNA
Project description:time-course salt stress experiment of model legume Medicago truncatula roots using Affymetrix Medicago Array, aimed to dig some useful gene for improve salt resistance for legumes and other crops Experiment Overall Design: 4 samples of Medicago truncatula germinated seedlings Experiment Overall Design: grown in Petri dishes for 3 days Experiment Overall Design: 180mM NaCl salt stress Experiment Overall Design: material collect at 0, 6, 24, 48 hour after salt stress beginning Experiment Overall Design: 3 biological replicates Experiment Overall Design: 2 ug of total RNA was used to synthesize doublestranded cDNA
Project description:In this study, proteomics was used to sequence the salt stress treatment group and the control group of Medicago sativa and Medicago truncatula. The aim was to discover the kegg pathway of the two alfalfa varieties under salt stress, which was of great significance to the exploration of the salt tolerance mechanism of alfalfa.
Project description:A Salt stress (100mM) was applied to root tips of Medicago truncatula (Jemalong A17 genotype) for 1h. Microarrays were used to compare the transcriptome of these plants (4 replicates) with the transcriptome of control plants, not submitted to the salt stress (4 replicates)