Project description:Environmental stress is detrimental to plants viability and requires an adequate reprogramming of cellular activities to maximize plant survival. We present a global analysis of the adaptive stress response of Arabidopsis thaliana to prolonged heat stress. We combine deep sequencing of RNA and ribosome protected fragments to provide genome wide map of adaptation to heat stress on at transcriptional and translational level. Our analysis shows that the genes with the highest upregulation upon heat stress are known heat-responsive gene, chaperons and other genes involved in protein folding control. Majority of these genes exhibits increase on both transcriptional and translational level. No translational inhibition or ribosome stalling was observed, which can be observed in the early thermal stress response, indicating that plants alter their cellular composition in order to adapt to the prolonged exposure to increased temperatures.
Project description:Plant hormones auxin and ethylene are the key regulators of plant growth and development, and their crosstalk is essential for numerous processes. Large-scale identification of new molecular targets in this crosstalk and their characterization is a tempting idea. To address this issue, we combined our own and publicly available Arabidopsis thaliana transcriptome data on auxin and ethylene treatments in a meta-analysis.
Project description:Global warming seriously threats world food supply. However, very few approaches have succeeded in genetically enhancing crop heat tolerance without growth penalty. To reveal the underlying molecular mechanism of Erecta action in response to thermal stress, we performed transcriptional profiling of Col-0 and mutant er-105 plants with or without 40℃ heat treatment on a global scale using the Affymetrix Arabidopsis ATH1 GeneChip.
Project description:Plant reproduction is one key biological process very sensitive to heat stress and, as a consequence, enhanced global warming imposes serious threats to sustain food safety worldwide. In this work we have focused on the molecular impact that high temperature conditions impose on gene expression of Arabidopsis pollen germinated in vitro. We have used a high-resolution ribosome profiling technology to provide, for the first time, a comprehensive study of how both the transcriptome and the translatome of germinated pollen respond to the increase in temperature. Although heat shock responses operate properly under high temperature conditions, we have uncovered important alterations under elevated temperature regimes down-regulating essential processes linked to cation/proton exchange and to carbohydrate/cation symport transport. These alterations provide molecular explanations to the dramatic alterations of pollen tube growth under heat stress. Overall a high correlation between transcriptional and translational responses to high temperature was found, but specific regulations at the translational level are also present in pollen subjected to temperature challenging conditions.
