Pediculus humanus corporis
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA18879 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:The human body louse is a vector for bacterial agents that cause louse-borne relapsing fever, trench fever, and epidemic typhus
| PRJNA16222 | ENA
Project description:Human body lice, Pediculus humanushumanus, are blood-feeding parasites that live in human clothing and feed several times per day.Saliva from louse bites induces local inflammation in the skin, pruiritis,and, if untreated, chronic Pediculosis has systemic negative health effects.Despite the well-established medical importance of body lice and their longstanding coevolution with humans, characterization of their saliva has been very limited.To address this, we extracted RNA and protein from two of the body louse’s morphologically distinct, sets of salivary glands (Bean-shaped and U-shaped) and generated transcript and protein profiles for each.Additionally, we performed fluorescent staining and confocal microscopy on each gland type to enhance descriptions of their morphology and detail their gross cellular architecture and arrangement.Analysis of the body louse salivary gene products and proteins revealed that the overwhelming majority were not closely related to biomolecules of known function; highlighting the organism’s unique and understudied saliva composition.Unexpectedly, and despite the contrasting morphology of the 2 gland types, there was ahigh degree of overlap in the salivary products produced.This finding suggests a strongDarwinian selection pressure to maintain both salivary glands types, given that it would seemingly be simpler to have a single morphologically homogeneous set of salivary glands.Here, we present the first next-generation sequencing and proteomic characterization of the human body louse sialome, discuss the potential physiological importance of louse salivary proteins, and present possible explanations for why lice have such a complex salivary gland organization despite ahigh degree of redundancy in protein repertoires between Bean and U-shaped salivary glands.
2025-02-19 | PXD058011 | Pride
Project description:Genome sequencing of Pediculus humanus corporis strain USDA
| PRJNA16223 | ENA
Project description:Basic helix-loop-helix (bHLH) proteins comprise a large superfamily of transcription factors, which are involved in the regulation of various developmental processes. bHLH family members are widely distributed in various eukaryotes including yeast, fruit fly, zebrafish, mouse, and human. In this study, we identified 55 bHLH motifs encoded in genome sequence of the human body louse, Pediculus humanus corporis (Phthiraptera: Pediculidae). Phylogenetic analyses of the identified P. humanus corporis bHLH (PhcbHLH) motifs revealed that there are 23, 11, 9, 1, 10, and 1 member(s) in groups A, B, C, D, E, and F, respectively. Examination to GenBank annotations of the 55 PhcbHLH members indicated that 29 PhcbHLH proteins were annotated in consistence with our analytical result, 8 were annotated different with our analytical result, 12 were merely annotated as hypothetical protein, and the rest 6 were not deposited in GenBank. A comparison on insect bHLH gene composition revealed that human body louse possibly has more hairy and E(spl) genes than other insect species. Because hairy and E(spl) genes have been found to negatively regulate the differentiation of insect preneural cells, it is suggested that the existence of additional hairy and E(spl) genes in human body louse is probably the consequence of its long period adaptation to the relatively dark and stable environment. These data provide good references for further studies on regulatory functions of bHLH proteins in the growth and development of human body louse.
| S-EPMC5634135 | biostudies-literature
Project description:Lack of paternal silencing and ecotype-specific expression in head and body lice hybrids.
| PRJNA968062 | ENA
Project description:Pediculus humanus corporis strain:Frisco Transcriptome or Gene expression
| PRJNA975842 | ENA
Project description:Illumina Sequencing of Pediculus Humanus Mitochondrial Coding region
| PRJNA179337 | ENA
Project description:Louse-borne diseases are prevalent in the homeless, and body louse eradication has thus far been unsuccessful in this population. We aim to develop a rapid and robust genotyping method usable in large field-based clinical studies to monitor permethrin resistance in the human body louse Pediculus humanus corporis. We assessed a melting curve analysis genotyping method based on real-time PCR using hybridization probes to detect the M815I-T917I-L920F knockdown resistance (kdr) mutation in the paraorthologous voltage-sensitive sodium channel (VSSC) α subunit gene, which is associated with permethrin resistance. The 908-bp DNA fragment of the VSSC gene, encoding the α subunit of the sodium channel and encompassing the three mutation sites, was PCR sequenced from 65 lice collected from a homeless population. We noted a high prevalence of the 3 indicated mutations in the body lice collected from homeless people (100% for the M815I and L920F mutations and 56.73% for the T917I mutation). These results were confirmed by melting curve analysis genotyping, which had a calculated sensitivity of 100% for the M815I and T917I mutations and of 98% for the L920F mutation. The specificity was 100% for M815I and L920F and 96% for T917I. Melting curve analysis genotyping is a fast, sensitive, and specific tool that is fully compatible with the analysis of a large number of samples in epidemiological surveys, allowing the simultaneous genotyping of 96 samples in just over an hour (75 min). Thus, it is perfectly suited for the epidemiological monitoring of permethrin resistance in human body lice in large-scale clinical studies.
| S-EPMC3405629 | biostudies-literature
Project description:Pediculus humanus capitis Genome sequencing
| PRJNA837756 | ENA
Project description:Insect reproduction is extremely variable, but the implications of alternative genetic systems are often overlooked in studies on the evolution of insecticide resistance. Both ecotypes of Pediculus humanus (Phthiraptera: Pediculidae), the human head and body lice, are human ectoparasites, the control of which is challenged by the recent spread of resistance alleles. The present study conclusively establishes for the first time that both head and body lice reproduce through paternal genome elimination (PGE), an unusual genetic system in which males transmit only their maternally derived chromosomes. Here, we investigate inheritance patterns of parental genomes using a genotyping approach across families of both ecotypes and show that heterozygous males exclusively or preferentially pass on one allele only, whereas females transmit both in a Mendelian fashion. We do however observe occasional transmission of paternal chromosomes through males, representing the first known case of PGE in which whole-genome meiotic drive is incomplete. Finally, we discuss the potential implications of this finding for the evolution of resistance and invite the development of new theoretical models of how this knowledge might contribute to increasing the success of pediculicide-based management schemes.
| S-EPMC5947629 | biostudies-literature