Dataset Information

Anolis carolinensis strain:JBL SC #1

ABSTRACT: The green anole is a lizard of the southeastern United States

PROVIDER: PRJNA18887 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Dataset's files

Source:

Items per page:

1 - 5 of 440

Similar Datasets

Project description:Protemics of anolis carolinensis tails undergoing regeneration. As amniote vertebrates, lizards are the most closely related organisms to humans capable of appendage regeneration. Lizards can autotomize, or release their tails as a means of predator evasion, and subsequently regenerate a functional replacement. Green anoles (Anolis carolinensis) can regenerate their tails through a process that involves differential expression of hundreds of genes, which has previously been analyzed by transcriptomic and microRNA analysis. To investigate protein expression in regenerating tissue, we performed whole proteomic analysis of regenerating tail tip and base. This is the first proteomic data set available for the green anole lizard. We identified 976 proteins only in the regenerating tail base, 796 only in the tail tip, and 874 in both tip and base. For 90% of these proteins in these tissues, we were able to assign a clear orthology to gene models in either the Ensembl or NCBI databases. For 20 proteins in the tail base (2.5%), 7 proteins in the tail tip (0.9%), and 7 proteins in both regions (0.8%), the gene model in Ensembl and NCBI matched an uncharacterized protein, confirming that these predictions are present in the proteome. Ontology and pathways analysis of proteins expressed in the regenerating tail base identified categories including actin filament-based process, ncRNA metabolism, regulation of phosphatase activity, small GTPase mediated signal transduction, and cellular component organization or biogenesis. Analysis of proteins expressed in the tail tip identified categories including regulation of organelle organization, regulation of protein localization, ubiquitin-dependent protein catabolism, small GTPase mediated signal transduction, morphogenesis of epithelium, and regulation of biological quality. These proteomic findings confirm pathways and gene families activated in tail regeneration in the green anole as well as identify uncharacterized proteins whose role in regrowth remains to be revealed.

			Action	DRS
		Other
	DRR001153.fastq.gz	Fastqsanger.gz
	DRR001154.fastq.gz	Fastqsanger.gz
	DRR001157.fastq.gz	Fastqsanger.gz
	DRR001158.fastq.gz	Fastqsanger.gz

Dataset Information

Anolis carolinensis strain:JBL SC #1

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets