Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Mus musculus

ABSTRACT: DNA methylation of hematopoietic stem cells during ontogeny and after enforced proliferative history

PROVIDER: PRJNA188939 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR691400.fastq.gz	Fastqsanger.gz
	SRR691401.fastq.gz	Fastqsanger.gz
	SRR691402.fastq.gz	Fastqsanger.gz
	SRR691403.fastq.gz	Fastqsanger.gz
	SRR691404.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 25

Similar Datasets

DNA methylation of hematopoietic stem cells during ontogeny and after enforced proliferative history

Project description:Genome-scale DNA methylation profiles at nucleotide resolution covering the vast majority of CpG islands and a representative sampling of conserved non-coding elements, transposons and other genomic features generated using high-throughput reduced representation bisulfite sequencing (RRBS) for murine hematopoietic stem cells during ontongeny and after enforced prolferative history. Analysis of hematopoietic stem cell methylation during ontogeny and after undergoing enforced proliferative history

2013-02-14 | E-GEOD-44117 | biostudies-arrayexpress

DNA methylation of hematopoietic stem cells during ontogeny and after enforced proliferative history

2013-02-14 | GSE44117 | GEO

Proliferation-dependent alterations of the DNA methylation landscape underlie hematopoietic stem cell aging

Project description:Gene Expression profiling of HSCs isolated at different stages of ontogeny to address correlation between gene expression and changes in DNA methylation Gene expression profiling of hematopoietic stem cells isolated from e14.5 fetal liver (lineage- ckit+ sca1+CD150+ CD48- Mac1+), 3 month old mice (lineage- ckit+ sca1+ CD34- Flk2-) and 25 month old mice (lineage- ckit+ sca1+ CD34- Flk2-)

2013-02-14 | E-GEOD-43729 | biostudies-arrayexpress

Mus musculus

Project description:The transcriptional landscape of hematopoietic stem cell ontogeny

| PRJNA157527 | ENA

Proliferative stress in hematopoietic stem cells partially drives their functional decline during aging by DNA methylation imprints

Project description:Proliferative stress in hematopoietic stem cells partially drives their functional decline during aging by DNA methylation imprints

| PRJNA1146326 | ENA

Expression data from developing hematopoietic stem and progenitor cells

Project description:In the mouse embryo during midgestation, hematopoietic stem cells (HSCs) are generated from aortic endothelium through the transdifferentiation process known as endothelial-to-hematopoietic transition, or EHT. During EHT, flat-shaped hemogenic endothelium transform into round-shaped hematopoietic cells, which subsequently form cell aggregates, called hematopoietic clusters. To understand HSC specification during ontogeny and to search for nascent HSC markers, we performed single-cell microarray analysis of developing HSC populations.

2019-05-01 | GSE113892 | GEO

Genome-Wide DNA Methylation Profiles in Hematopoietic Stem and Progenitor Cells Reveal Over-Representation of ETS Transcription Factor Binding Sites

Project description:DNA methylation is an essential epigenetic mark that is required for normal development. Knockout of the DNA methyltransferase enzymes in the mouse hematopoietic compartment reveals that methylation is critical for hematopoietic differentiation. To better understand the role of DNA methylation in hematopoiesis, we characterized genome-wide DNA methylation in primary mouse hematopoietic stem cells (HSC), common myeloid progenitors (CMP), and erythroblasts (ERY). Methyl Binding Domain protein 2 (MBD) enrichment of DNA followed by massively-parallel sequencing (MBD-Seq) was used to map genome-wide DNA methylation. Globally, DNA methylation was most abundant in HSC, with a 40% reduction in CMP, and 67% reduction in ERY. Only 3% of peaks arise during differentiation demonstrating a genome-wide decline in DNA methylation during erythroid development. Analysis of genomic features revealed that 98% of promoter CpG islands are hypomethylated, while 20-25% of non-promoter CpG islands are methylated. Proximal promoter sequences of expressed genes are hypomethylated in all cell types, while gene body methylation positively correlates with gene expression in HSC and CMP. Elevated genome-wide DNA methylation in HSC and the positive association between methylation and gene expression demonstrates that DNA methylation is a mark of cellular plasticity in HSC. Utilizing de novo motif discovery we identified overrepresented transcription factor consensus binding motifs in methylated sequences. Motifs for several ETS transcription factors, including GABPalpha and ELF1 are overrepresented in methylated regions. Our genome-wide survey demonstrates that DNA methylation is markedly altered during myeloid differentiation and identifies critical regions of the genome and transcription factor programs that contribute to hematopoiesis. Examination of changes in methylation profiles during hematopoietic stem cell differentiation

2012-06-01 | E-GEOD-38354 | biostudies-arrayexpress

Mus musculus

Project description:de novo DNA Methylation Balances Hematopoietic Stem Cell Self-Renewal and Differentiation

| PRJNA137177 | ENA

Transcriptome analysis of nascent hematopoietic cells

Project description:During ontogeny, hematopoietic stem cells (HSCs) and erythroid-myeloid progenitors (EMPs) are generated from distinct hemogenic endothelium. Nascent HSCs and EMPs are distinguished by the expression of Hlf. To obtain mechanistic insight into HSC specification, we compared the gene expression profiles between Hlf+ and Hlf- nascent hematopoietic cells.

2019-05-03 | GSE114164 | GEO

Transcriptional regulatory network controlling the ontogeny of hematopoietic stem cells [scRNA-seq]

Project description:Transcriptional regulatory network controlling the ontogeny of hematopoietic stem cells.

2020-05-06 | GSE146684 | GEO