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ABSTRACT: Gene expression profiling of zebrafish embryos at 5 days post fertilization

OTHER RELATED OMICS DATASETS IN: PXD000145

PROVIDER: PRJNA189535 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR726540.fastq.gz	Fastqsanger.gz
	SRR726541.fastq.gz	Fastqsanger.gz
	SRR726542.fastq.gz	Fastqsanger.gz

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2013-08-01 | E-GEOD-44351 | biostudies-arrayexpress

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Project description:We compared Agilent custom made expression microarrays with Illumina deep sequencing for RNA analysis of zebrafish embryos 5 days post fertilization, showing as expected a high degree of correlation of expression of a common set of 15,927 genes. This microarray study was designed to determine the gene expression profile of zebrafish embryos 5 days post fertilization. We also have compared expression with embryos that were injected with Mycobacterium marinum in the yolk at 2 hours post fertilization. After injections embryos were transferred into fresh egg water and incubated at 28M-BM-0C. 150 embryos of mock-injected embryos or 200 embryos injected with 12 CFU bacteria were snap-frozen in liquid nitrogen, and total RNA was isolated using TRIZOL reagent. The samples were analyzed in a technical duplicate using a dye swap experiment in order to check for dye bias.

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Gene expression profiling of zebrafish embryos at 5 days post fertilization [Agilent Arrays]

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