Project description:To elucidate the mechanisms involved in pulmonary inflammation in Zaire ebolavirus-infected pigs, lung sections infected pigs were used for a porcine microarray to analyse changes in gene expression with particular interest in proinflammatory cytokine and chemokine transcription.
Project description:To elucidate the mechanisms involved in pulmonary inflammation in Zaire ebolavirus-infected pigs, lung sections infected pigs were used for a porcine microarray to analyse changes in gene expression with particular interest in proinflammatory cytokine and chemokine transcription. Pigs were given inhalation anaesthesia and then inoculated with 106 plaque forming units of Zaire ebolavirus in sterile PBS per pig via the intranasal, intraocular and oral routes. Three control pigs were mock inoculated with PBS and housed separately.
Project description:This study evaluates a resequencing microarray designed to determine the sequence of the four major genes in the Ebolavirus genome, Nucleoprotein (NP), matrix protein (VP40), glycoprotein (GP) and polymerase (L). The array has the ability to determine the sequence of five species and strains: Zaire Ebolavirus (Mayinga and Makona), Bundibugyo Ebolavirus, Sudan Ebolavirus and Tai Forest Ebolavirus. Illumina Next Generation Sequencing verified the sequence of the Zaire Ebolavirus (Mayinga) sample.
Project description:Large White and Meishan pigs were either non-treated or injected with mammalian 1-24 ACTH (Immediate Synachten, Novartis France) at the dose of 250 µg per animal. Pigs were sacrificed either immediately after capture from their home cage (non-treated animals) or 1 hour following ACTH injection. Adrenal glands were immediately collected from pigs and frozen on dry ice and then stored at -80°C until RNA isolation. Keywords: stress response, adrenal, gene expression, pig
Project description:This study evaluates a resequencing microarray designed to determine the sequence of the four major genes in the Ebolavirus genome, Nucleoprotein (NP), matrix protein (VP40), glycoprotein (GP) and polymerase (L). The array has the ability to determine the sequence of five species and strains: Zaire Ebolavirus (Mayinga and Makona), Bundibugyo Ebolavirus, Sudan Ebolavirus and Tai Forest Ebolavirus.
