Alcanivorax borkumensis SK2
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA190900 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:A. borkumensis cells: pyruvate vs. cells grown on hexadecane, canola and diesel
| PRJNA217898 | ENA
Project description:Transcriptional profiling of Alcanivorax borkumensis cells, grown on either pyruvate or hexadecane as carbon source, that were stressed with 1- octanol. The gene expression was measured 15 min, 30 min, 60 min and 90 min after 1-octanol addition. Two-condition experiment, 1-octanol-stressed vs. unstressed cells; different time points were investigated after 1-octanol was spiked in (15 min, 30 min, 60 min, 90 min); for each condition 3 replicates were used, for the control condition 4 replicates (pyruvate cultures) and 3 replicates (hexadecane cultures) were used for analysis
2013-02-27 | E-GEOD-44687 | biostudies-arrayexpress
Project description:Produces extracellular glucose-lipids which may be used for reducing the effect of oil spills.
| PRJNA13005 | ENA
Project description:Transcriptional profiling of Alcanivorax borkumensis cells, grown on either pyruvate or hexadecane as carbon source, that were stressed with 1- octanol. The gene expression was measured 15 min, 30 min, 60 min and 90 min after 1-octanol addition.
2013-02-27 | GSE44687 | GEO
Project description:Alcanivorax borkumensis isolate:ABS183 Genome sequencing and assembly
| PRJNA296541 | ENA
Project description:Alcanivorax borkumensis strain:JGI ETNP_300m_187_B10 Genome sequencing
| PRJNA247869 | ENA
Project description:Alcanivorax is a hydrocarbonoclastic genus dominating oil spills worldwide. While its presence has been detected in oil-polluted seawaters, marine sediment and salt marshes under ambient pressure, its presence in deep-sea oil-contaminated environments is negligible. Recent laboratory studies highlighted the piezosensitive nature of some Alcanivorax species, whose growth yields are highly impacted by mild hydrostatic pressures (HPs). In the present study, osmotic stress was used as a tool to increase HP resistance in the type strain Alcanivorax borkumensis SK2. Control cultures grown under standard conditions of salinity and osmotic pressure with respect to seawater (35.6 ppt or 1136 mOsm kg(-1), respectively) were compared with cultures subjected to hypo- and hyperosmosis (330 and 1720 mOsm kg(-1), or 18 and 62 ppt in salinity, equivalent to brackish and brine waters, respectively), under atmospheric or increased HP (0.1 and 10 MPa). Osmotic stress had a remarkably positive impact on cell metabolic activity in terms of CO2 production (thus, oil bioremediation) and O2 respiration under hyperosmosis, as acclimation to high salinity enhanced cell activity under 10 MPa by a factor of 10. Both osmotic shocks significantly enhanced cell protection by reducing membrane damage under HP, with cell integrities close to 100% under hyposmosis. The latter was likely due to intracellular water-reclamation as no trace of the piezolyte ectoine was found, contrary to hyperosmosis. Notably, ectoine production was equivalent at 0.1 MPa in hyperosmosis-acclimated cells and at 10 MPa under isosmotic conditions. While stimulating cell metabolism and enhancing cell integrity, osmotic stress had always a negative impact on culture growth and performance. No net growth was observed during 4-days incubation tests, and CO2:O2 ratios and pH values indicated that culture performance in terms of hydrocarbon degradation was lowered by the effects of osmotic stress alone or combined with increased HP. These findings confirm the piezosensitive nature of A. borkumensis, which lacks proper resistance mechanisms to improve its metabolic efficiency under increased HP, thus explaining its limited role in oil-polluted deep-sea environments.
| S-EPMC4870253 | biostudies-literature
Project description:Glycine-glucolipid, a glycolipid, is natively synthesized by the marine bacterium Alcanivorax borkumensis SK2. A. borkumensis is a Gram-negative, non-motile, aerobic, halophilic, rod-shaped γ-proteobacterium, classified as an obligate hydrocarbonoclastic bacterium. Naturally, this bacterium exists in low cell numbers in unpolluted marine environments, but during oil spills, the cell number significantly increases and can account for up to 90% of the microbial community responsible for oil degradation. This growth surge is attributed to two remarkable abilities: hydrocarbon degradation and membrane-associated biosurfactant production. This study aimed to characterize and enhance the growth and biosurfactant production of A. borkumensis, which initially exhibited poor growth in the previously published ONR7a, a defined salt medium. Various online analytic tools for monitoring growth were employed to optimize the published medium, leading to improved growth rates and elongated growth on pyruvate as a carbon source. The modified medium was supplemented with different carbon sources to stimulate glycine-glucolipid production. Pyruvate, acetate, and various hydrophobic carbon sources were utilized for glycolipid production. Growth was monitored via online determined oxygen transfer rate in shake flasks, while a recently published hyphenated HPLC-MS method was used for glycine-glucolipid analytics. To transfer into 3 L stirred-tank bioreactor, aerated batch fermentations were conducted using n-tetradecane and acetate as carbon sources. The challenge of foam formation was overcome using bubble-free membrane aeration with acetate as the carbon source. In conclusion, the growth kinetics of A. borkumensis and glycine-glucolipid production were significantly improved, while reaching product titers relevant for applications remains a challenge.
| S-EPMC10710537 | biostudies-literature
Project description:Transcriptional profiling of Alcanivorax borkumensis cells, grown on either pyruvate or hexadecane, canola and diesel as carbon source. Two-condition experiment, cells grown on pyruvate vs. cells grown on hexadecane, canola or diesel. For each condition 4 replicates were used, as for the control condition also 4 replicates were used for analysis.
2014-07-01 | E-GEOD-50528 | biostudies-arrayexpress