Project description:Renibacterium salmoninarum is a Gram-positive, intracellular bacterial pathogen that causes Bacterial Kidney Disease (BKD) in Atlantic salmon (Salmo salar). To identify R. salmoninarum bacterin-responsive genes in Atlantic salmon, fish (n=6) were intraperitoneally injected with a high (5×107 cells/kg; High-Rs) dose of formalin-killed R. salmoninarum or phosphate-buffered saline (PBS control). Head kidney samples were collected at 24 h post-injection. Using 44K microarray analysis, transcriptome analysis was performed to identify differentially expressed probes in response to formalin-killed R. salmoninarum injection.
Project description:Renibacterium salmoninarum is a Gram-positive, intracellular bacterial pathogen that causes Bacterial Kidney Disease (BKD) in Atlantic salmon (Salmo salar). To identify Atlantic salmon genes responsive to R. salmoninarum pathogen, fish were intraperitoneally injected with R. salmoninarum (BKD) or sterile KDM-2 medium (control). Head kidney samples were collected at 13 days post-infection. Using 44K microarray analysis, transcriptome analysis was performed to identify differentially expressed probes in response to different levels of R. salmoninarum infection.
Project description:Proteomic analysis of iron restriction of the marine pathogen Renibacterium salmoninarum that causes the disease (BKD), in this study two isolated Chilean H-2 strains and the type strain ATCC33209 have been used. These results show that changes are generated at the level of protein expression in pathways involved in iron metabolism and uptake.
Project description:This study used subcellular fractionation combined with proteomics for a comprehensive overview of the protein localizations of R. salmoninarum proteins within enriched subcellular fractions of cytosolic, membrane, extracellular and membrane vesicle (MV) proteins. With this approach, 1,674 protein groups were label-free quantified and the abundance of proteins involved in virulence processes were compared between the subcellular fractions.
