Project description:Microarray analysis of peripheral blood mononuclear cells (PBMCs) from IgA nephropathy patients, membranous nephropathy patients and healthy controls.
Project description:The current study makes use of NanoString targeted technology to profile urinary exosomal miRNAs from IgA nephropathy affected patients and corresponding healthy controls. Circulatory biomarkers were detected for IgA nephropathy from Indian cohort which can be made used for the diagnostic therapy. 14 miRNAs were detected to be related to the disregulation in miRNome of IgA nephropathy patients using lasso feature selection method, out of which multiple miRNAs like hsa.mir.146b.3p, hsa.mir.599 and many more was resulted with high AUROC values >=0.9 efficient in differentiating between healthy controls and IgA nephropathy condition. These markers can be use further in the diagnosis and treatment of IgAN.
Project description:The basic defect of IgA nephropathy (IgAN) lies within peripheral blood mononuclear cells rather than local kidney abnormalities. Previously we showed an altered gene expression in monocytes compared to B and T cells isolated from IgAN patients (Kidney Int, 2010), thus our aim here was to study this subset more closely at genome-wide level.
Project description:The current study makes use of NanoString targeted technology to profile urinary exosomal miRNAs from IgA nephropathy affected patients and corresponding healthy controls. In addition to IgA nephropathy, disease controls belonging to the condition of Lupus nephritis, Focal segmental glomerulosclerosis, Diabetic nephropathy, Minimal change disease, Membranous nephropathy and Hypertensive nephropathy were also assayed for their miRNA expression profile. In addition to the circulatory biomarkers detected for IgA nephropathy from Indian cohort, their validation for being specific to IgAN was performed by adding negative control samples which can be made used for the diagnostic therapy. These provided miRNA markers specific to IgAN and other markers related to IgAN.
Project description:Expression data from human with IgA nephropathy (IgAN) and hypertensive nephropathy (HT) We used microarrays to analyze the transcriptome of microdissected renal biopsies from patients with IgAN and HT
Project description:Expression data from human with IgA nephropathy (IgAN) and hypertensive nephropathy (HT) We used microarrays to analyze the transcriptome of microdissected renal biopsies from patients with IgAN and HT RNA from glomeruli and tubulointerstitial compartments was extracted and processed for hybridization on Affymetrix microarrays.
