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Sus scrofa

ABSTRACT: Whole transcriptome analyses of three Berkshire and three Korean native pig fat, liver and muscle tissue using RNA-Seq

PROVIDER: PRJNA193204 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR776497_1.fastq.gz	Fastqsanger.gz
	SRR776497_2.fastq.gz	Fastqsanger.gz
	SRR776498_1.fastq.gz	Fastqsanger.gz
	SRR776498_2.fastq.gz	Fastqsanger.gz
	SRR776499_1.fastq.gz	Fastqsanger.gz

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Project description:The pig could be a useful model to characterize molecular aspects determining several delicate phenotypes because they have been bred for those characteristics. The Korean native pig (KNP) is a regional breed in Korea that was characterized by relatively high intramuscular fat content and reddish meat color compared to other western breeds such as Yorkshire (YS). YS grew faster and contained more lean muscle than KNP. We compared the KNP to Yorksire to find molecular clues determining muscle characteristics. The comparison of skeletal gene expression profiles between these two breeds showed molecular differences in muscle. We found 82 differentially expressed genes (DEGs) defined by fold change (more than 1.5 fold difference) and statistical significance (within 5% of false discovery rate). Functional analyses of these DEGs indicated up-regulation of most genes involved in cell cycle arrest, down-regulation of most genes involved in cellular differentiation and its inhibition, down-regulation of most genes encoding component of muscular-structural system, and up-regulation of most genes involved in diverse metabolism in KNP. Especially, DEGs in above-mentioned categories included a large number of genes encoding proteins directly or indirectly involved in p53 pathway. Our results indicated a possible role of p53 to determine muscle characteristics between these two breeds. Experiment Overall Design: Comparing gene expression profiles to discover differentially expressed genes from skeletal muscles of two different pig breeds.

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