Thelohanellus kitauei
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA193360 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| Other | ||||
| SRR1103279.fastq.gz | Fastqsanger.gz | |||
| SRR17152144_1.fastq.gz | Fastqsanger.gz | |||
| SRR17152144_2.fastq.gz | Fastqsanger.gz | |||
| SRR17152863_1.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 5 of 24 |
Similar Datasets
Project description:Whole genome sequencing of Thelohanellus kitauei
| PRJEB53887 | ENA
Project description:Thelohanellus kitauei transcriptome sequencing for evolutionary and phylogenetic analyses (Illumina Hiseq 4000)
| PRJNA786276 | ENA
Project description:Thelohanellus kitauei transcriptome sequencing for evolutionary and phylogenetic analyses (Illumina Hiseq 2500)
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Project description:Thelohanellus kitauei isolate:Wuqing Genome sequencing and assembly
| PRJNA193083 | ENA
Project description:Thelohanellus kitauei, is a member of obligate parasitic myxozoans, which causes intestinal giant-cystic disease of common carp (Cyprinus carpio) and has resulted in significant economic losses in carp farms. Cystatin secreted by parasites can regulate the immune response of host to facilitate parasite's survival. In this study, the secretory TK-cystatin gene, encoding a protein of 120 amino acid residues (13.65 kDa), was cloned from T. kitauei genome. Phylogenetic analysis showed that TK-cystatin gene is closely related to the cystatin-A from Hydra vulgaris. Multiple sequence alignment revealed that TK-cystatin had three conserved motifs: N-terminal G19G20, Q73VVAG77, and C-terminal L102P103. Molecular docking between TK-cystatin and three cysteine proteases showed a lower binding energy (- 13 KJ/mol) with cathepsin L whereas a higher binding energy (- 8.6 KJ/mol) with cathepsin B. TK-cystatin gene was expressed in Escherichia coli. Activity assays revealed that TK-cystatin has stronger inhibitory activity on endopeptidases (papain and cathepsin L) and weaker inhibitory activity on exopeptidase (cathepsin B). TK-cystatin was stable under the condition of acidity or alkalinity or below 57 °C. This study laid a foundation for the design and development of the anti-T. kitauei vaccine in carp culture in the future.
| S-EPMC7311561 | biostudies-literature
Project description:Members of Myxozoa, a parasitic metazoan taxon, have considerable detrimental effects on fish hosts and also have been associated with human food-borne illness. Little is known about their biology and metabolism. Analysis of the genome of Thelohanellus kitauei and comparative analysis with genomes of its two free-living cnidarian relatives revealed that T. kitauei has adapted to parasitism, as indicated by the streamlined metabolic repertoire and the tendency toward anabolism rather than catabolism. Thelohanellus kitauei mainly secretes proteases and protease inhibitors for nutrient digestion (parasite invasion), and depends on endocytosis (mainly low-density lipoprotein receptors-mediated type) and secondary carriers for nutrient absorption. Absence of both classic and complementary anaerobic pathways and gluconeogenesis, the lack of de novo synthesis and reduced activity in hydrolysis of fatty acids, amino acids, and nucleotides indicated that T. kitauei in this vertebrate host-parasite system has adapted to inhabit a physiological environment extremely rich in both oxygen and nutrients (especially glucose), which is consistent with its preferred parasitic site, that is, the host gut submucosa. Taking advantage of the genomic and transcriptomic information, 23 potential nutrition-related T. kitauei-specific chemotherapeutic targets were identified. This first genome sequence of a myxozoan will facilitate development of potential therapeutics for efficient control of myxozoan parasites and ultimately prevent myxozoan-induced fish-borne illnesses in humans.
| S-EPMC4986447 | biostudies-literature
Project description:Thelohanellus kitauei is a spore-forming myxosporean parasite prevalent in scattered mirror carp (Cyprinus carpio) that generates numerous cysts in the intestine and causes mass mortality in fish. To investigate the infection and mortality induced by T. kitauei in pond-reared farms in Luo-Jiang (104°51'N, 31°31'E), southwest China, morphological and molecular analyses of infected fish were conducted. Natural and specific immune indicators were further evaluated to determine the immunological effects of response to parasitic infection. The infectious parasite was identified as Thelohanellus kitauei based on morphological, 18S rDNA and infectious characteristics. Scattered mirror carp was determined as the specific intermediate host of the parasite. However, T. kitauei still caused considerable damage to the fish, in particular, injury and blockage of the intestines, resulting in malnutrition and even death. The mature spores of T. kitauei colonize the intestinal submucosa of carp and form cysts of various sizes that block the intestinal tract and release spores into the enteric cavity upon rupture, leading to the next phase of T. kitauei growth. Moreover, T. kitauei-infected carp showed weaker innate immunity. IgM is involved in the fight against parasitic infection while cytokines, such as IL-6, IL-1β and TNF-α, had an impact on infection processes. To our knowledge, this is the first report to show that T. kitauei infects and causes death in scattered mirror carp. Our collective findings from systematic pathology, morphology and immunology experiments provide a foundation for further research on infections by this type of parasite and development of effective treatment strategies.
| S-EPMC6934683 | biostudies-literature
Project description:Intestinal giant-cystic disease (IGCD) of the Israel carp (Cyprinus carpio nudus) has been recognized as one of the most serious diseases afflicting inland farmed fish in the Republic of Korea, and Thelohanellus kitauei has been identified as the causative agent of the disease. Until now, studies concerning IGCD caused by T. kitauei in the Israel carp have been limited to morphological and histopathological examinations. However, these types of diagnostic examinations are relatively time-consuming, and the infection frequently cannot be detected in its early stages. In this study, we cloned the full-length 18S rRNA gene of T. kitauei isolated from diseased Israel carps, and carried out molecular identification by comparing the sequence with those of other myxosporeans. Moreover, conventional PCR and real-time quantitative PCR (qPCR) using oligonucleotide primers for the amplification of 18S rRNA gene fragment were established for further use as methods for rapid diagnosis of IGCD. Our results demonstrated that both the conventional PCR and real-time quantitative PCR systems applied herein are effective for rapid detection of T. kitauei spores in fish tissues and environmental water.
| S-EPMC3375447 | biostudies-literature
Project description:Thelohanellus Kudo, 1933 is a species rich genus of Myxosporea, sharing many morphological similarities with species of Myxobolus but the former possesses a single polar capsule, and the latter has two. Based on molecular phylogenetic analyses, this single distinguishing feature is not monophyletic, and members of Thelohanellus are intermixed with Myxobolus species, calling into question the validity of genus Thelohanellus. The occurrence of two polar capsules in a small proportion of Thelohanellus spores as observed in this study suggests that these species have the capacity to express this Myxobolus-like trait, clouding the distinction of these two genera further. Herein, using the most comprehensive data set to date, we explored the phylogenetic relationships of Thelohanellus to other myxobolids, to investigate the evolutionary history of the genus Thelohanellus and the origins of single polar capsule in this group. The phylogenetic analyses and statistical tests of topology revealed Thelohanellus as a strongly supported polyphyletic lineage, clustering in five distinct branches within Myxobolus clade. Ancestral state reconstruction for polar capsule number showed that Thelohanellus species have evolved from myxosporean species with two polar capsules at least four times, which could be classified in three possible evolutionary pathways. The polyphyly of Thelohanellus and the multiple evolutionary origins of single polar capsule of Thelohanellus demonstrate that the distinction of this genus from Myxobolus is largely for convenience, and does not reflect their evolutionary history.
| S-EPMC6319017 | biostudies-literature
Project description:A genomic overview of in vivo binding of a transcription factor Otx in the ascidian early gastrula embryo.
2010-03-09 | GSE17690 | GEO