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Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

ABSTRACT: Screening for novel immunogenic proteins of Campylobacter jejuni NCTC 11168

PROVIDER: PRJNA193420 | ENA |

REPOSITORIES: ENA

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Screening for novel immunogenic proteins of Campylobacter jejuni NCTC 11168

Project description:The screening of a cDNA derived expression library of Campylobacter jejuni NCTC 11168 expressed in E. coli using a fusion construct and specific HaloTag interaction to a modified surface is shown. 1536 different clones were screened including positive (hisJ, cjaA, peb1a) and negative (argC, pyrC, gapA) reference proteins. The goal of the screening was to identify potential novel immunogenic proteins from C. jejuni by selecting clones showing a high signal intensity in comparison to the known antigens used as positive markers. Afterwards, the most promising clones were sequenced to identify the gene and corresponding protein, and these proteins were then investigated further. Consequently, 22 novel immunogenic proteins could be identified.

2013-04-06 | GSE45268 | GEO

Differential RNA-sequencing of Campylobacter jejuni NCTC 11168

Project description:We report the use of differential RNA-sequencing for the determination of the primary transcriptome of wildtype Campylobacter jejuni NCTC 11168. This allows for the genome-wide determination of transcription start sites.

2013-09-13 | GSE49312 | GEO

RNA-sequencing of Campylobacter jejuni NCTC 11168 wildtype and fur perR mutant

2014-07-01 | GSE49687 | GEO

RNA-sequencing of Campylobacter jejuni NCTC 11168 wildtype and fur perR mutant

Project description:We report the use of RNA-seq analysis for the determination of RPKM transcript levels in wildtype and fur perR mutant of Campylobacter jejuni NCTC 11168. This allows for comparison of gene expression levels. Campylobacter jejuni NCTC 11168 wildtype and fur perR mutant were grown to late log phase, RNA was purified and used for RNA-sequencing by Illumina HiSeq sequencing

2014-07-01 | E-GEOD-49687 | biostudies-arrayexpress

Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

Project description:Epitope mapping of potential novel immunogenic proteins of Campylobacter jejuni NCTC 11168

| PRJNA194601 | ENA

Transcriptional profiles of Campylobacter jejuni NCTC 11168 to 4mg/L erythromycin treatment

Project description:Erythromycin is the drug of choice to treat campylobacteriosis, but resistance to this antibiotic is rising. The adaptive mechanisms employed by Campylobacter jejuni to erythromycin treatment remain unknown. The aim of this study is to determine the molecular basis underlying CampylobacterM-bM-^@M-^Ys immediate response to Ery treatment. The design utilized an available two color microarray slide for the entire transcriptome of Campylobacter jejuni wild type strain NCTC 11168. One hybridizations were performed: sham-treated NCTC 11168 v.s. lethal dose erythromycin treated NCTC 11168. Samples were independently grown and harvested. There were three biological replicates of each sample.

2013-10-10 | E-GEOD-43023 | biostudies-arrayexpress

Transcriptional profiles of Campylobacter jejuni NCTC 11168 to 0.125mg/L erythromycin treatment

Project description:Erythromycin is the drug of choice to treat campylobacteriosis, but resistance to this antibiotic is rising. The adaptive mechanisms employed by Campylobacter jejuni to erythromycin treatment remain unknown. The aim of this study is to determine the molecular basis underlying CampylobacterM-bM-^@M-^Ys immediate response to Ery treatment. The design utilized an available two color microarray slide for the entire transcriptome of Campylobacter jejuni wild type strain NCTC 11168. One hybridizations were performed: sham-treated NCTC 11168 v.s. sub-lethal dose erythromycin treated NCTC 11168. Samples were independently grown and harvested. There were three biological replicates of each sample.

2013-10-10 | E-GEOD-43024 | biostudies-arrayexpress

Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

Project description:Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819 Variation

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Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

Project description:Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819 Genome sequencing

| PRJNA85567 | ENA

Genomic and Proteomic Characterization of Sessile and Planktonic Campylobacter jejuni NCTC 11168

Project description:In order to understand the cellular mechanisms that facilitate a surface-associated lifestyle, expression profiles were determined at the levels of transcription and translation for sessile and planktonic Campylobacter jejuni NCTC 11168 (obtained from American Type Culture Collection (ATCC 700819)). These investigations indicate that the immobilized bacteria undergo a shift in cellular priorities away from metabolic, motility and protein synthesis capabilities towards emphasis on iron uptake, oxidative stress defense and membrane transport. Keywords: transcript profiling

2005-07-29 | GSE3028 | GEO

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