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Penicillium decumbens

ABSTRACT: Transcriptomic analysis of fungus Penicillium decumbens and brlA deletion strains in different culture medium

PROVIDER: PRJNA196504 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR822377.fastq.gz	Fastqsanger.gz
	SRR822378.fastq.gz	Fastqsanger.gz
	SRR822379.fastq.gz	Fastqsanger.gz
	SRR822380.fastq.gz	Fastqsanger.gz

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Transcriptomic analysis of fungus Penicillium decumbens and brlA deletion strains in different culture medium

Project description:Transcriptomic analysis of fungus Penicillium decumbens and brlA deletion strains in liquid medium and solid medium respectivelly Examination of differential gene expressions by Penicillium decumbens strains 114-2 and brlA deletion stains in liquid medium and solid medium

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Transcriptomic analysis of fungus Penicillium decumbens and brlA deletion strains in different culture medium

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Transcriptomic analysis of cellulolytic fungus Penicillium decumbens strains in response to different carbon sources

Project description:Digital gene expression profiling (DGE) was used to compare the responses of Penicillium decumbens strains to different carbon sources including glucose, cellulose and cellulose-wheat bran. In both wild-type strain 114-2 and cellulase hyperproducing mutant JU-A10-T, transcription of lignocellulolytic enzymes were significantly up-regulated in the presense of cellulose. Relative to 114-2, coordinated up-regulation of lignocellulolytic enzymes and down-regulation of amylases and proteases were observed in JU-A10-T, especially in the cellulose-wheat bran medium. The expression of the principal β-glucosidase BGLI gene was not elevated in JU-A10-T, like the cellulases and hemicellulases, suggesting a different regulatory mechanism for this enzyme. Functional analysis of genes up-regulated in JU-A10-T relative to 114-2 also showed enrichment of proteins involved in amino acid synthesis, protein synthesis, and post-translational modification, compatible with the higher level of production of secreted proteins in JU-A10-T.

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Transcriptomic analysis of fungus Penicillium oxalicum and its laeA deletion strains in 24h and 60h

Project description:The goals of this study are to compare differential gene expressions for Penicillium oxalicum wild type strain (WT), and laeA knockout strain (ΔlaeA) in different development phase. The deletion of laeA downregulated genes involved in oxidation- reduction process, alkaloid metabolic process, and transmembrane transport. We find the expression levels of seven secondary metabolism gene clusters (totally 28 clusters) were silenced inΔlaeA. This study provides the information that laeA function are required in conidiation and hydrolase activity of P. oxalicum. Examination of differential gene expressions by digital gene expression tag profiling in Penicillium oxalicum wild type strain and laeA knockout mutant strains in 24h and 60h in modified Czapek culture medium with 2% glucose as carbon resource. qRT–PCR validation was performed using SYBR Green assays.

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