Project description:The aim of this study was to identify differently expressed genes between first and last runs of serial repitchings from four different German breweries. In the breweries the number of successive runs varied from 4 to 22. A run describes the industrial fermentation of brewery wort. By the end of fermentation the yeast slurry is cropped. These cells are reused for inoculation of another cylindro-conical vessel (CCVs).
Project description:The aim of this study was to identify differently expressed genes between first and last runs of serial repitchings from four different German breweries. In the breweries the number of successive runs varied from 4 to 22. A run describes the industrial fermentation of brewery wort. By the end of fermentation the yeast slurry is cropped. These cells are reused for inoculation of another cylindro-conical vessel (CCVs). Results from eight microarrays are summarized in this study. The samples were taken from four German breweries. Microarrays were hybridized in a dye swap approach: Dye-swap 1: brewery A runs 1 vs. 22; Dye-swap 2: brewery B runs 1 vs. 5; Dye-swap 3: brewery C runs 1 vs. 4; Dye-swap 4: brewery D runs 1 vs. 8
Project description:Industrial bioethanol production may involve a low pH environment,improving the tolerance of S. cerevisiae to a low pH environment caused by inorganic acids may be of industrial importance to control bacterial contamination, increase ethanol yield and reduce production cost. Through analysis the transcriptomic data of Saccharomyces cerevisiae with different ploidy under low pH stress, we hope to find the tolerance mechanism of Saccharomyces cerevisiae to low pH.
