Nasonia vitripennis
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ABSTRACT:
PROVIDER: PRJNA197232 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Nasonia Tiling Microarray Experiments for Expression Profiling Sexual Development (Gonads)
| PRJNA197231 | ENA
Project description:Nasonia Tiling Microarray Experiments for Expression Profiling Sexual Development (Pupa)
| PRJNA197233 | ENA
Project description:Nasonia Tiling Microarray Experiments for Expression Profiling Sexual Development (Larva)
| PRJNA197234 | ENA
Project description:Nasonia Tiling Microarray Experiments for Expression Profiling Sexual Development (Embryo18)
| PRJNA197235 | ENA
Project description:Nasonia Tiling Microarray Experiments for Expression Profiling Sexual Development (Embryo10)
| PRJNA197236 | ENA
Project description:Nasonia Tiling Microarray Experiments for Expression Profiling Sexual Development
| PRJNA197229 | ENA
Project description:Profiling of expression level in adult female Nasonia vitripennis
| PRJNA186077 | ENA
Project description:The expression level in the parasitoid Nasonia vitripennis adult female samples was profiled and compared with the methylation pattern. Methylated and non-methylated genes showed markedly different patterns. The expression level was higher for methylated than non-methylated genes. non-nmethylated genes account for 99% of the genes that were not found to be expressed in the adult female RNA-seq data (FPKM < 0.1). Unlike methylated genes, the expression distribution of the non-methylated genes was bimodal with a lower expressed group and a moderately expressed group of genes, indicating that methylation status is not the only determinant of high expression in adult females. Methylated genes also have lower coefficient of variation (CV) of expression level across five developmental stages, suggesting that they are expressed more evenly across development. Profiling of expression level in adult female Nasonia vitripennis by RNA-seq
2013-08-27 | E-GEOD-43422 | biostudies-arrayexpress
Project description:Whole Genome Bisulphite Sequencing of Nasonia vitripennis in the embryo, larval, prepupal, pupal and adult stages of development.
2024-12-16 | E-MTAB-14656 | biostudies-arrayexpress
Project description:The extraordinary range in the degree of sexual dimorphism (SD) among animal species is widely perceived to be caused in part by differences in patterns of sexual selection, but sex-specific adaptations and sex chromosome differences also play a role. Studies in insects have discovered a substantial number of sex-biased genes, but little is known about the epigenetic basis of SD. The degree and genome-wide distribution of sex-biased expression become interesting questions in hymenoptera species with haplodiploid sex-determination. To study the genetic and epigenetic architecture of SD and understand the conservation and evolution of sex-biased expression in a haplodiploid system that lacks sex chromosomes, we performed RNA-seq and whole-genome bisulfite sequencing in female and male adult samples of two parasitoid wasp species, Nasonia vitripennis and Nasonia giraulti. More than 75% of the expressed genes displayed significantly sex-biased expression. Both the number and the degree of sex-biased genes are higher than insects like Drosophila melanogaster, which have sex-chromosome mediated sex determination. Females from the two Nasonia species have far more similar expression profiles than does the contrast between the two sexes within either species. Interestingly, the extremely male- and female-biased genes are enriched for totally different functional categories: male-biased genes are highly enriched for key enzymes in sex-pheromone synthesis; female-biased genes are enriched for nuclear-located genes that are responsible for epigenetic regulation of gene expression. Unlike gene expression profiles, DNA methylomes are more similar within species, and no stable differentially methylated genes have been found between the two sexes, suggesting that DNA methylation is not directly responsible for the molecular basis of SD. However, methylation status does influence sex-biased expression: 80% of female-biased genes are methylated, which is more than two-fold higher than the genome average (30%); almost all male-biased and sex-specific genes are non-methylated, which is consistent with the fact that methylated genes have house-keeping functions and a broader expression breadth. Evolutionarily, male-biased genes have greater sequence divergence between the two species, and they are more likely to have a functional paralog in the Nasonia genome. Sex-specific genes have significantly higher non-synonymous substitution rates and dN/dS ratios. In addition, local clusters of sex-biased genes in the genome may have epigenetic properties similar to the sex chromosome. In summary, Nasonia accomplish a striking degree of sex-differential expression through a difference in ploidy along with associated differences in methylations status. Profiling of expression levels in Nasonia vitripennis and Nasonia giraulti adult male and female samples using Illumina RNA-seq
2015-06-08 | E-GEOD-61156 | biostudies-arrayexpress