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Carcinus maenas

ABSTRACT: shore or green crab, an invasive species of diverse marine and estuarine habitats

PROVIDER: PRJNA20117 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1564428_1.fastq.gz	Fastqsanger.gz
	SRR1564428_2.fastq.gz	Fastqsanger.gz
	SRR1572181_1.fastq.gz	Fastqsanger.gz
	SRR1572181_2.fastq.gz	Fastqsanger.gz
	SRR1586326_1.fastq.gz	Fastqsanger.gz

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Effects of elevated seawater pCO2 on gene expression patterns in the gills of the green crab, Carcinus maenas

2011-10-10 | GSE28870 | GEO

Carcinus maenas

Project description:Effects of elevated seawater pCO2 on gene expression patterns in the gills of the green crab, Carcinus maenas

| PRJNA140589 | ENA

Effects of elevated seawater pCO2 on gene expression patterns in the gills of the green crab, Carcinus maenas

Project description:In order to promote our understanding of the responses of green crab acid-base regulatory epithelia to high pCO2, Baltic Sea green crabs were exposed to a pCO2 of 400 Pa for 3 and 7 days after which posterior gills 7 and 9 were sampled. Gills were then subsequently screened for differentially expressed gene transcripts using a 4,462-feature microarray developed by Towle et al. 2010. For each experimental block (gill7-day3, gill7-day7, gill9-day3, gill9-day7), 6 replicate samples were obtained for control (= 39 Pa) and elevated (= 400 Pa) pCO2 exposed animals. Each microarray slide included 4 technical replicates for each transcript and was hybridized with one control pCO2 (labelled with AlexaFluor555) and one elevated pCO2 cDNA (labelled with AlexaFluor647). Lowess-normalized gene expression was calculated as the log2 of the ratio of the fluorescence intensity of the CO2-treatment cDNA to the fluorescence intensity of the control cDNA (log2 ratio=F635/F532).

2011-10-09 | E-GEOD-28870 | biostudies-arrayexpress

Carcinus maenas

Project description:Transcriptomic analysis of crustacean neuropeptide signalling throughout the moult cycle

| PRJNA400568 | ENA

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Project description:Carcinus maenas Raw sequence reads

| PRJNA283611 | ENA

Carcinus maenas

Project description:Carcinus maenas Transcriptome

| PRJNA255867 | ENA

Carcinus maenas

Project description:Carcinus maenas Raw sequence reads

| PRJNA377723 | ENA

Carcinus maenas

Project description:EST project located at the Mount Desert Island Biological Laboratory

| PRJNA20119 | ENA

European green crab (Carcinus maenas) gut

Project description:Diet of Green Crabs in New England Estuaries using Metagenomic Techniques

| PRJNA1114726 | ENA

Limitations of eDNA analysis for Carcinus maenas abundance estimations.

Project description:BackgroundEnvironmental DNA (eDNA) is an effective tool for the detection and monitoring of presence or absence of rare and invasive species. These techniques have been extended to quantify biomass in vertebrates, particularly in fish species. However, the efficacy of eDNA techniques to quantify biomass in invertebrate species has rarely been examined. This study tested whether eDNA could be used to determine the biomass of the world-wide invasive green crab, Carcinus maenas. In a controlled laboratory study, the relationship between biomass and C. maenas eDNA concentration was examined in the context of different biotic (activity) and abiotic (temperature) parameters.ResultsWhen incubating different numbers of crabs in sterile saltwater for up to 7 days, a relationship between eDNA concentration and biomass was observed at temperatures of 6.7 ℃ and 18.7 ℃, but not at 12.8 ℃. Additionally, motor activity, aggression level, time of sampling, and features of organismal decay had significant impact on the concentration of C. maenas eDNA collected.ConclusionsWe show that eDNA concentration did not correlate with biomass, and that biomass, temperature, organismal characteristics, and potentially many more parameters affect shedding and degradation rates for eDNA in this species, thus, impacting the recoverable eDNA concentration. Therefore, eDNA techniques are not likely to provide a reliable signal of biomass in the invasive invertebrate species C. maenas.

| S-EPMC8822865 | biostudies-literature

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