Mus musculus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA202098 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Macrophages initiate, modulate, and also serve as final effector cells in immune responses during course of schistosomal infections. Presently, we discussed the roles of the gene expression profile and functional changes of macrophages in immune responses against the Schistosoma japonicum by microarray experiments. Hierarchical clustering analysis demonstrated that a significant switch in gene transformation associated with a type-1 response and linked with a type-2 cytokine phenotype occurs between 4.5 and 8 weeks post-infection. Moreover, the gene profiles at 3 later time-points following egg challenge were similar in complexity and magnitude. These data also showed that there are mostly inhibition in gene expression related TLR, IFN, MHC and TNFrsf at the switch between 4.5 and 8 weeks post-infection, It is suggested that these immunomodulatory genes may be down-regulated in resistance against S. japonicum eggs and granuloma pathology. The induction of alternatively activated macrophage was important for dampening the inflammation in hepatic granulomas and contributing to a decrease in cytotoxicity. The genes expressions involved in repair/remodeling during liver fibrosis were also observed after eggs production. Understanding these immune mechanisms related to parasite resistance, pathology, and growth with regard to the disease will be helpful in further studies on S. japonicum. Two-condition experiment, Control mice vs. Schistosoma japonicum-infected mice. replicates: 2 infected replicates. Different post-infection weeks
2013-05-09 | E-GEOD-46719 | biostudies-arrayexpress
Project description:Rats and mice were infected with Schistosoma japonicum, and the worms were collected from infected rats and mice. Worms in rats were exposed to high level of NO, while the worms in mice were not. Then a S-nitrosocysteine proteomics of Schistosoma japonicum collected from infected rats and mice was performed and the data were collected.
2024-04-19 | PXD041667 |
Project description:We have used a Schistosoma japonicum infected murine model with in vivo sub-lethal dosages of praziquantel against adult parasites. Differential gene expression of parasites was followed between 30 minutes and 24 hours post- drug administration, using a whole transcriptome microarray platform. Differential gene expression was considered separately between parasite gender. Total RNA was isolated from adult (7 week post cercarial challenge) Schistosoma japonicum male and females. Gene expression was determined through hybridisation on an Agilent custom designed oligo microarray.
2012-09-27 | E-GEOD-41149 | biostudies-arrayexpress
Project description:We have used a Schistosoma japonicum infected murine model with in vivo sub-lethal dosages of praziquantel against adult parasites. Differential gene expression of parasites was followed between 30 minutes and 24 hours post- drug administration, using a whole transcriptome microarray platform. Differential gene expression was considered separately between parasite gender.
2012-09-27 | GSE41149 | GEO
Project description:DNA microarrays were used to profile gene expression in normal murine splenic cells vs. prion infected splenic cells with the aim of identifying host factors involved in prion propagation and/or useful as biomarkers for early diagnosis. Two condition experiment, prion infected murine splenic cells vs. normal murine splenic cells: 4 infected biological replicates, 2 control biological replicates, 2 technical replicates (dye-swaps) for each competitive hybridization. Samples were run on 4 different arrays: MPB Murine 16K BMAP-MSV printrun 7.1 microarray MPB Murine 16K BMAP-MSV printrun 9.1 microarray MPB Murine 32K Oligo printrun 4.1 microarray Agilent 4x44K Whole Mouse Genome
2011-07-01 | E-GEOD-17446 | biostudies-arrayexpress
Project description:Murine chondrocytes: Control vs Ad-Epas1 infected
| PRJNA297520 | ENA
Project description:It is well recognized that parasitic helminth infections, which afflict more than one billion people globally, correlate with a decreased prevalence of metabolic diseases, including obesity and type 2 diabetes, but the molecular mechanisms involved remain to be determined. Using microarrays, we quantified the temporal gene expression profiles in the liver of Schistosoma japonicum-infected C57BL/6 mice at 9 weeks post infection with that from uninfected mice as controls. More than 150 miRNAs were differentially expressed in the liver during S. japonicum infection, and miRNA-mRNA network would provide new evidence for the negtive correlation between S. japonicum infection and metabolism.
2019-07-26 | GSE134866 | GEO
Project description:Revealing the reproductive mechanism of schistosomes will help to control this disease. In this study, the proteomic profiles of single-sex infected female (SF) worms and bisexual infected mature female (MF) worms of Schistosoma japonicum at 18, 21, 23 and 25 days post infection (dpi) were identified with isobaric tags for relative and absolute quantitation-coupled liquid chromatography-tandem mass spectrometry. Differentially expressed proteins (DEPs) were subsequently used for bioinformatic analysis.
2024-01-26 | PXD030834 | Pride
Project description:Background: Schistosomiasis remains an important global public health problem that affects 285 million people in 78 countries. The immune response mechanisms of host–parasite interaction are complex, and miRNAs play a major role in modulation of development, homeostasis, as well as the function of immune cells. Results: In this study, an miRNA microarray was applied to investigate differences in miRNA expression in splenic B cells of normal and 13w post infected mice. In total, 43 miRNAs were detected in splenic B cells of the C57BL/6 mice before and after infection, including 33 miRNAs with up-regulated expression, and 10 miRNAs with down-regulated expression in mice 13w infection with schistosomes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of the differentially expressed miRNAs revealed that many important biological pathways are triggered by schistosome infection in C57BL/6 mice, such as the Thyroid hormone signal pathway, Axon guidance, insulin signaling pathway, Pathways in cancer and Rap1 signaling pathway. The results reveal that miRNAs may be an important regulator of splenic B cells in the chronic phase of Schistosoma japonicum infection. Conclusions: The data presented here provide valuable information to increase understanding of the basic role of miRNAs in splenic B cells during the interaction of the S. japonicum with the host. This may be helpful in identifying the upstream molecular pathways controlling B cell specialization and provides a new platform of B cell manipulation to fine-tune their function.
2021-06-01 | GSE115443 | GEO