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Gossypium hirsutum

ABSTRACT: cotton Gene expression profiles deciphering leaf senescence variation between early- and late-senescence cotton lines

PROVIDER: PRJNA202936 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR3536226.fastq.gz	Fastqsanger.gz
	SRR3536227.fastq.gz	Fastqsanger.gz
	SRR867611.fastq.gz	Fastqsanger.gz

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Global gene expression analysis of cotton (Gossypium hirsutum L.) under drought stress in leaf tissue.

Project description:Transcriptome analysis in cotton under drought stress. To study the molecular response of drought stress in cotton under field condition global gene expression analysis was carried out in leaf tissue. Gossypium hirsutum cv. Bikaneri Nerma was used for the gene expression analysis. Cotton plants were subjected to drought stress at peak flowering stage. Leaf samples were collected when the soil moisture content was 19.5% which is 50% of the normal control plots. Gene expression profiles in drought induced and their respective control samples were analyzed using Affymertix cotton Genechip Genome arrays to study the global changes in the expression of genome.

2012-06-04 | GSE29566 | GEO

Global gene expression analysis of cotton (Gossypium hirsutum L.) under drought stress in leaf tissue.

2012-06-03 | E-GEOD-29566 | biostudies-arrayexpress

Identification of anther expressing genes in cotton (Gossypium hirsutum)

Project description:This study was initiated with the objective of identifying the anther/tapetum specific promoters from cotton floral buds. Cotton is an important commercial crop. Hybrid cotton varieties are developed to obtain improved yield and fiber quality. Most of the hybrid seed production in cotton is carried out by hand emasculation, which requires large amount of manpower, resulting in high cost of hybrid seed. We are developing barnase-barstar based male sterility system, which would be a better alternative for hybrid development. The tapetum specific promoters are main requirement for such a system. The study was thus carried out to identify genes expressed in the anthers. Cotton bud sizes were correlated with tapetum development. RNA was isolated from following tissues: • Anther tissues from buds at pre-meiotic stage of development (Tapetum absent) • Buds without anther tissues at pre-meiotic stage of development • Anther tissues from buds during meiosis (Tapetum present) • Buds without anther tissues during meiosis • Anther tissues from buds at post-meiotic stage of development (Tapetum degenerated) • Buds without anther tissues at post-meiotic stage of development • Leaf tissues • Seedling 5 days after germination Biotin labeled cRNA was hybridized on Affymertix cotton Genechip Genome array following Affymetrix protocols. Three biological replicates were maintained.

2013-10-31 | E-GEOD-51779 | biostudies-arrayexpress

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Project description:Expression data from the process of chilling stress causing Alternaria alternata infection and leading to cotton leaf senescence

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High-resolution survey of transcriptome from Wild type Croker 312 and Phytochrome-A1D (PhyA1D) RNAi cotton (Gossypium hirsutum) lines

Project description:Cotton (Gossypium hirsutum) is the major contributor of feedstock for the fabric industry and thus building genomic resources in cotton such as this study are a way to understand the cotton plant's biology. Cotton cultivars that suppress PHYA1D (PhyA1 homeolog on the D genome of a tetraploid) exhibit early-flowering, increased fiber length and increased seed yield. In our proposed study, flower buds (also called squares) samples were collected from control (Croker 312 wildtype line) and RNAi lines carrying the PhyA1D suppression. RNA samples from the two lines including three biological replicates were subjected to RNA-seq sequencing to elucidate the transcriptome profile.

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Global gene expression analysis of cotton (Gossypium hirsutum L.) under drought stress in leaf tissue and during fibre development stages.

Project description:This SuperSeries is composed of the following subset Series: GSE29566: Global gene expression analysis of cotton (Gossypium hirsutum L.) under drought stress in leaf tissue. GSE29567: Global gene expression analysis of cotton (Gossypium hirsutum L.) under drought stress during fibre development stages. Refer to individual Series

2012-06-03 | E-GEOD-29810 | biostudies-arrayexpress

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Project description:The development of viable gametes is especially susceptible to heat in all higher plants, including cotton (Gossypium hirsutum cv. Sicot 71), resulting in substantial reduction in lint quantity and quality at temperatures above 32 °C. Male reproductive cells are especially vulnerable to heat. This study demonstrates a relatively small impact of heat on leaves compared with a profound impact during early and late male gametophyte development. To investigate the mechanisms leading to heat sensitivity, the proteome of pollen was analyzed after two distinct phases of development (tetrads or binucleate microspores) had been exposed for 5 d to 36/25 °C (day/night) or 40/30 °C. The resulting mature pollen grains were collected for quantitative label-free shotgun proteomic analysis. A total of 868 proteins was quantified. Interestingly, Hsp70s were highly induced in response to extreme heat indicating key roles of this family to cope with heat stress.

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