Project description:Somatic embryogenesis (SE) is an ideal model for plant cell totipotency. Transition from somatic cells to embryonic cells is the key to SE. The poor frequency of embryonic callus (EC) induction has limited the application of SE in many plants, such as Agapanthus praecox. We performed large-scale, quantitative proteomic and metabolomic analyses with different callus differentiation directions (SE and organogenesis) and stages (initial SE and repetitive SE) to better understand the morphological, physiological, and molecular characteristics of the acquisition of embryogenic ability in A. praecox.