Project description:Parasitic infections with the hookworms Ancylostoma ceylanicum and Necator americanus are a significant global health burden and current hookworm infection management approaches are limited by modest drug efficacy, costs, risk of reinfection and drug resistance. Subunit vaccines based on proteins excreted and secreted (ES) by hookworms that impart sufficient efficacy to reduce worm numbers and associated disease burden is a promising management strategy to overcome these limitations. However, existing studies on the ES proteome of hookworms have mainly described proteins derived from the adult life stage which may preclude the opportunity to target larvae-specific parasitic processes. In this project, we use high resolution mass spectrometry to identify and compare ES proteins from the L3 stage as well as the adult stage of N. americanus and A. ceylanicum respectively.
Project description:These data belong to a metabolic engineering project that introduces the reductive glycine pathway for formate assimilation in Cupriavidus necator. As part of this project we performed short-term evolution of the bacterium Cupriavidus necator H16 to grow on glycine as sole carbon and energy source. Some mutations in a putiative glycine transporting systems facilitated growth, and we performed transcriptomics on the evolved strain growing on glycine. Analysis of these transcriptomic data lead us to the discovery of a glycine oxidase (DadA6), which we experimentally demonstrated to play a key role in the glycine assimilation pathay in C. necator.