Project description:We analyzed the changes in the brain tissue of Apis mellifera ligustica at the molecular level by sequencing after using fluvalinate. We found that the differentially expressed miRNAs (DEM) may be involved in hippocampal cell apoptosis and damage to memory functions. This result may be related to behaviors observed after the administration of this medication, such as a lack of homing at night and behavioral disturbances. Overall, our results provide new information about the molecular mechanisms and pathways of fluvalinate action in the brain tissue of Apis mellifera ligustica.
Project description:A.mellifera ligustica with similar group potential and randomly divided them into two sets (3 replicates in each group). One set as control groups, and the other set as test groups. The test groups were exposed to fluvalinate at a normal dosage for administration to honeybees (according to the instructions for use of the medicine, mite treatment was added every 7 days). The sequencing is performed by the Illumina HiSeq TM 4000.