Project description:Timecourse of gene expression changes in Drosophila SoxN homozygous mutant embryos compared with their heterozygous siblings, from stage 7 to 13 of embryonic development.
Project description:Timecourse of gene expression changes in Drosophila SoxN homozygous mutant embryos compared with their heterozygous siblings, from stage 7 to 13 of embryonic development. Five time points: stage 7-8, stage 9, stage 10, stage 11 and stage 12-13. Four biological replicates per time point. Two conditions: SoxN homoxygous vs SoxN heterozygous mutant embryos.
Project description:Expression profiling of Drosophila Lmd (lame-duck) homozygous mutant embryos in a timecourse of embryogenesis. Five one-hour timepoints were assayed (5-11 hrs after egg laying). Homozygous mutant embryos were collected using GFP-activated embryo sorting at the same time as stage-matched wildtype controls. Four independent collections were performed at each timepoint. Total RNA was extracted and amplified. Mutant and respective wildtype samples were hybridized together on cDNA arrays.
Project description:Expression profiling of Drosophila twist homozygous mutant embryos in a timecourse of embryogenesis. Four one-hour timepoints were assayed (4-8 hrs after egg laying). Homozygous mutant embryos were collected using GFP-activated embryo sorting at the same time as stage-matched wildtype controls. Four independent collections were performed at each timepoint. Total RNA was extracted and amplified. Mutant and respective wildtype samples were hybridized together to cDNA arrays.
Project description:Expression profiling of D. melanogaster biniou homozygous mutant embryos in a timecourse of embryogenesis. Five one-hour timepoints were assayed (5-10 hrs, 13-14 hrs after egg laying). Homozygous mutant embryos were collected using GFP-activated embryo sorting at the same time as stage-matched wildtype controls. Four independent collections were performed at each timepoint. Total RNA was extracted and amplified. Mutant and respective wildtype samples were hybridized together on INDAC oligo-arrays.
Project description:Expression profiling of Drosophila Mef2 homozygous mutant embryos in a timecourse of embryogenesis. Twelve one-hour timepoints were assayed (5-16 hrs, 18-19 hrs after egg laying). Homozygous mutant embryos were collected using GFP-activated embryo sorting at the same time as stage-matched wildtype controls. Four independent collections were performed at each timepoint. Total RNA was extracted and amplified. Mutant and respective wildtype samples were hybridized together on cDNA arrays.
