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Comparative analysis of the response to iron-limitation in the absence of oxygen in R. sphaeroides

ABSTRACT: Comparative analysis of the response to iron-limitation in the absence of oxygen in R. sphaeroides

PROVIDER: PRJNA205626 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR868777.fastq.gz	Fastqsanger.gz
	SRR868778.fastq.gz	Fastqsanger.gz
	SRR868779.fastq.gz	Fastqsanger.gz
	SRR868780.fastq.gz	Fastqsanger.gz

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Comparative analysis of the response to iron-limitation in the absence of oxygen in R. sphaeroides [Microarray]

Project description:High intracellular levels of unbound iron can contribute to the production of reactive oxygen species (ROS) in the Fenton reaction, while depletion of iron limits the availability of iron containing proteins, some of which have important functions in the oxidative stress defense. Vice versa increased ROS levels lead to damage of proteins with iron sulfur centers. Thus organisms have to coordinate and balance their responses to oxidative stress and iron availability. Our knowledge on the molecular mechanisms underlying the coregulation of these responses is still limited. To discriminate between a direct cellular response to iron limitation and indirect responses, which are the consequence of increased levels of ROS, we compared the response of the alpha proteobacterium Rhodobacter sphaeroides to iron limitation in presence or absence of oxygen. While some genes respond to iron limitation exclusively or much stronger in presence of oxygen, other genes show much stronger response in anaerobic conditions. Remarkably few genes show even opposite response to iron depletion in presence or absence of iron.

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Comparative analysis of the response to iron-limitation in the absence of oxygen in R. sphaeroides [Microarray]

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Comparative analysis of the response to iron-limitation in the presence or absence of oxygen in R. sphaeroides and role of the OxyR protein [RNA-Seq]

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Comparative analysis of the response to iron-limitation in the presence or absence of oxygen in R. sphaeroides and role of the OxyR protein [RNA-Seq]

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R. sphaeroides Δirr -Fe vs. R. sphaeroides Δirr +Fe under microaerobic conditions

Project description:Transcriptional profiling of R. sphaeroides Δirr under iron limitation (-Fe) compared to control R. sphaeroides Δirr under normal growth conditions (+Fe).

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Project description:Transcriptional profiling of R. sphaeroides pBBRUpsMx2 compared to control R. sphaeroides pBBR under high oxygen and singlet oxygen conditions.

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R. sphaeroides Δirr -Fe vs. R. sphaeroides Δirr +Fe under microaerobic conditions

Project description:Transcriptional profiling of R. sphaeroides Δirr under iron limitation (-Fe) compared to control R. sphaeroides Δirr under normal growth conditions (+Fe). Two strain experiment under normal iron (+Fe) and iron limitation (-Fe) conditions. 6 Biological replicates, independently grown and harvested at OD660=0,4; 1-3 pooled in replicate 1, 4-6 pooled in replicate 2

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Project description:Transcriptional profiling of R. sphaeroides Δlov compared to control R. sphaeroides 2.4.1 under singlet oxygen stress, aerobic conditions

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Project description:Comparative analysis of the response to iron-limitation in the absence of oxygen in R. sphaeroides [Microarray]

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Project description:Transcriptional profiling of R. sphaeroides pRKPcrZ compared to control R. sphaeroides pRK4352 under low oxygen conditions. In this study we compared the transcriptome of a PcrZ over-expression strain to a control strain. PcrZ is the first small non-coding RNA, which is involved in the complex regulatory network of photosynthesis gene regulation in Rhodobacter sphaeroides. It counteracts the formation of photosynthetic complexes in a redox dependent manner.

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