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Phlebiopsis gigantea

ABSTRACT: Phlebiopsis gigantea overview

PROVIDER: PRJNA208104 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR1045110.fastq.gz	Fastqsanger.gz
	SRR1045111.fastq.gz	Fastqsanger.gz
	SRR1045104.fastq.gz	Fastqsanger.gz
	SRR1045105.fastq.gz	Fastqsanger.gz

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Mechanism of extractive degradation by Phlebiopsis gigantea

Project description:Mass spectrometric analysis of extracellular proteins in culture filtrate was performed to elucidate the mechanisms of extractive degradation by Phlebiopsis gigantea of microcrystalline cellulose coated and uncoated with an acetone extract from the Pinus taeda (loblolly pine)

2020-09-14 | PXD021332 | Pride

Comparisons of Phlebiopsis gigantea transcript profiles when cultured different substrates

Project description:Collectively classified as white-rot fungi, certain basidiomycetes efficiently degrade the major structural polymers of wood cell walls. A small subset of these Agaricomycetes, exemplified by Phlebiopsis gigantea, is capable of colonizing freshly exposed conifer sapwood despite its high pitch content, which retards the establishment of other fungal species. The mechanism(s) by which P. gigantea tolerates and metabolizes resinous compounds have not been explored. Here, we report the annotated P. gigantea genome and compare profiles of its transcriptome and secretome when cultured on fresh-cut versus solvent-extracted loblolly pine. The P. gigantea genome contains a conventional repertoire of hydrolase genes involved in cellulose/hemicellulose degradation, whose patterns of expression were relatively unperturbed by the absence of extractives. The expression of genes typically ascribed to lignin degradation was also largely unaffected. In contrast, genes likely involved in the transformation and detoxification of pitch were highly induced in its presence. Their products included an ABC transporter, lipases, cytochrome P450s, glutathione S-transferase and aldehyde dehydrogenase. Other regulated genes of unknown function and several constitutively expressed genes are also likely involved in P. gigantea’s pitch metabolism. These results contribute to our fundamental understanding of conifer colonization and carbon cycling processes. Phlebiopsis gigantea was cultivated in media containing one of three carbon sources: freshly harvested loblolly pine (3 replicates), acetone extracted lobollly pine (3 replicates), or glucose (2 replicates). RNA was extracted and processed for Illumina sequencing as described below.

2014-10-01 | E-GEOD-53112 | biostudies-arrayexpress

Comparisons of Phlebiopsis gigantea transcript profiles when cultured different substrates

2014-10-01 | GSE53112 | GEO

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