Project description:We sequenced the mRNA in the Y-organ of normal and eyestalk-ablated Eriocheir Sinensis and obtained the transcriptome profiling (RNA-seq) of the two samples with Next-generation sequencing (NGS) technique. The unigene expression of the two samples were compared and the differetially expressed unigenes were listed.
Project description:The transcriptome profiling five tissues of juvenile Eriocheir sinensis, including gill, muscle, thoracic ganglion, eyestalk and hepatopancreas, were sequenced to get the basic dataset for constructed a genome-scale metabolic network model for E. sinensis. The model was used to predict the optimal nutrient requirements of E. sinensis in feed and suggestions for feed improvement were put forward based on the simulation results.