Project description:Genome-wide transcriptomics (RNA-seq) data was obtained temporally at 0, 15, 30, 45, 60 and 120 minutes of the infection with phage 18:3 on Cellulophaga baltica strain #18 to analyze, in biological triplicates, the phage and host transcriptional response during their interaction compared to the uninfected control.
Project description:We performed high-througput RNA sequencing of RNA samples extracted from C. baltica cells infected with phi14:2 phage at 40, 90, 140 and 190 minutes after infection. The same experiments were performed with cells treated with host RNA polymerase inhibitor in order to determine which genes rely on viral rifampicin-insensitive RNA polymerase. Using this data, we identified different time classes of phage genes and revealed genes transcribed by phage RNA polymerase.
