Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Synthetic plasmid

ABSTRACT: Synthetic plasmid Genome sequencing

PROVIDER: PRJNA209332 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR921384_1.fastq.gz	Fastqsanger.gz
	SRR921384_2.fastq.gz	Fastqsanger.gz
	SRR921439_1.fastq.gz	Fastqsanger.gz
	SRR921439_2.fastq.gz	Fastqsanger.gz
	SRR921440_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 6

Similar Datasets

Synthetic plasmid

Project description:Synthetic plasmid Raw sequence reads

| PRJNA282549 | ENA

Synthetic plasmid

Project description:Synthetic plasmid Raw sequence reads

| PRJNA1102096 | ENA

Synthetic plasmid

Project description:Synthetic plasmid Raw sequence reads - CORALINA: A simple method for the generation of gRNA libraries covering complete genomes

| PRJNA317714 | ENA

Synthetic plasmid

Project description:DSB-induced transcription of pLac-Tet plasmid in cell-free extracts.

| PRJNA304447 | ENA

Plasmid pKJK172

Project description:Plasmid pKJK172 Genome sequencing and assembly

| PRJNA432895 | ENA

Plasmid pED208

Project description:Plasmid pED208 Genome sequencing and assembly

| PRJNA871772 | ENA

Plasmid vector pSP189

Project description:Plasmid vector pSP189 Genome sequencing

| PRJNA302825 | ENA

synthetic construct

Project description:synthetic construct Genome sequencing

| PRJNA877268 | ENA

Plasmid pENVA

Project description:Plasmid pENVA

| PRJEB5291 | ENA

A synthetic control genome provides a universal molecular standard to measure DNA, mRNA and protein expression

Project description:The phi X 174 bacteriophage was first sequenced in 1977, and has since become the most widely used standard in molecular biology and next-generation sequencing. However, with the advent of affordable DNA synthesis and de novo gene design, we considered whether we could engineer a synthetic genome, termed SynX, specifically tailored for use as a universal molecular standard. The SynX genome encodes 21 synthetic genes that can be in vitro transcribed to generate matched mRNA controls, and in vitro translated to generate matched protein controls. This enables the use of SynX as a matched control to compare across genomic, transcriptomic and proteomic experiments. The synthetic genes provide qualitative controls that measure sequencing accuracy across k-mers, GC-rich and repeat sequences, as well as act as quantitative controls that measure sensitivity and quantitative accuracy. We show how the SynX genome can measure DNA sequencing, evaluate gene expression in RNA sequencing experiments, or quantify proteins in mass spectrometry. Unlike previous spike-in controls, the SynX DNA, RNA and protein controls can be independently and sustainably prepared by recipient laboratories using common molecular biology techniques, and widely shared as a universal molecular standard.

2024-03-22 | PXD035035 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data