Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression profile changes during blastocysts hatching of embryos

ABSTRACT: Gene expression profile changes during blastocysts hatching of embryos

PROVIDER: PRJNA209520 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Gene expression profile changes during blastocysts hatching of embryos

Project description:This SuperSeries is composed of the SubSeries listed below.

2016-06-19 | GSE48292 | GEO

Late-term turkey embryo hatching muscle (complexus muscle) gene expression

Project description:Turkey embryos are very sensitive to perturbations in energy metabolism because they have a wider hatching window than chicken embryos. Mortality of turkey embryos during late-term incubation is high relative to chickens, and many surviving hatchlings have compromised vitality. Intestinal maturation at hatch is also crucial to survival and post-hatch performance. The study of poultry embryo metabolism during the last stages of incubation is difficult due to many shifts and changes that occur in preparation for hatching. Microarray technology is suitable to study complex biological systems like avian late-term embryonic development. Therefore, the objectives of this study were to create a customized focused oligonucleotide microarray based on chicken genome sequences that could be used to study late-term avian metabolism and intestinal maturation, and use this array to survey turkey embryos gene expression from 20 days of incubation until hatch. The key features of this microarray are that all genes present have been annotated and gene spot replication (4) within each array chip. Microarray analysis was performed on liver, pectoral muscle, hatching muscle, and duodenum Keywords: time course, embryo development

2008-01-31 | GSE9473 | GEO

Hatching is modulated by microRNA-378a-3p derived from extracellular vesicles secreted by blastocysts

Project description:Hatching is modulated by microRNA-378a-3p derived from extracellular vesicles secreted by blastocysts

| PRJNA812556 | ENA

Vitrification of pig embryos dysregulates the microRNA transcriptome profile

Project description:The aim of this study was to investigate the effects of vitrification on the miRNA transcriptome profile of porcine blastocysts and to compare the effects of the SOPS and Cryotop methods on miRNA profiles. The interactions of the miRNA data with previous gene expression data (Gonzalez-Plaza et al., 2023) from the same samples were also investigated. Embryos at the blastocyst stage (n=180) were selected for the experiment and divided into three experimental groups: blastocysts vitrified with the OC system (n=60), blastocysts vitrified with the SOPS system (n=60), and a control group consisting of fresh, nonvitrified blastocysts (n=60). After in vitro culture for 24 h, five pools of 8 viable embryos (n=40 embryos per group) were prepared for transcriptome analysis. Embryos were placed in sterile tubes with 5 µL of PBS, immediately immersed in LN2 and stored at -80 °C until microarray analysis.

2024-02-28 | E-MTAB-13814 | biostudies-arrayexpress

Gene expression profile changes during blastocysts hatching of embryos produced from parthenogenesis and in vivo

Project description:Comparative transcriptomic analyses among in vivo (IVV) XB, in vivo (IVV) HB, parthenogenetic activation (PA) XB, and parthenogenetic activation (PA) HB were performed following a reference design.

2016-06-19 | GSE48290 | GEO

Sus scrofa

Project description:Gene expression profile changes during blastocysts hatching of embryos produced from parthenogenesis and in vivo

| PRJNA209524 | ENA

Late-term turkey embryo liver, pectoralis muscle, hatching muscle and duodenum gene expression

2008-01-31 | GSE9475 | GEO

Hatching is modulated by microRNA-378a-3p derived from extracellular vesicles secreted by blastocysts (mRNA)

Project description:Hatching is modulated by microRNA-378a-3p derived from extracellular vesicles secreted by blastocysts (mRNA)

| PRJNA812576 | ENA

Hatching is modulated by microRNA-378a-3p derived from extracellular vesicles secreted by blastocysts (miRNA)

Project description:Hatching is modulated by microRNA-378a-3p derived from extracellular vesicles secreted by blastocysts (miRNA)

| PRJNA812577 | ENA

Transcriptomic alterations induced by physiologically relevant level of mono(2-ethylhexyl) phthalate (MEHP) in oocytes and blastocysts

Project description:Mono(2-ethylhexyl) phthalate (MEHP), the main di(2-ethylhexyl) phthalate (DEHP) metabolite, is a known reproductive toxicant. Residual levels of 20 nM MEHP have been found in follicular fluid aspirated from IVF-treated women and DEHP-treated animals. It is not yet clear whether these residual MEHP levels have any effect on the follicle-enclosed oocyte or developing embryo. To clarify this point, bovine oocytes were matured with or without 20 nM MEHP for 22 h. Microarray analysis was performed for both mature oocytes and 7-day blastocysts. A feasibility examination was performed on mature oocytes (n = 200/group) to reveal a possible direct effect on the oocyte proteomic profile. Transcriptome analysis revealed MEHP-induced alterations in the expression of 456 and 290 genes in oocytes and blastocysts, respectively. The differentially expressed genes are known to be involved in various biological pathways, such as transcription process, cytoskeleton regulation and metabolic pathway. Among these, the expression of 9 genes was impaired in both oocytes exposed to MEHP (i.e., direct effect) and blastocysts developed from those oocytes (i.e., carryover effect). In addition, 191 proteins were found to be affected by MEHP in mature oocytes. The study explores, for the first time, the risk associated with exposing oocytes to physiologically relevant MEHP concentrations to the maternal transcripts. Although it was the oocytes that were exposed to MEHP, alterations carried over to the blastocyst stage, following embryonic genome activation, implying that these embryos are of low quality.

2019-05-09 | PXD012092 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data