Saccharomyces cerevisiae strain:Bread strain, Wine strain, Bioethanol strain
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA212389 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR941816.fastq.gz | Fastqsanger.gz | |||
| SRR941817.fastq.gz | Fastqsanger.gz | |||
| SRR941818.fastq.gz | Fastqsanger.gz | |||
| SRR941819.fastq.gz | Fastqsanger.gz | |||
| SRR941821.fastq.gz | Fastqsanger.gz |
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Project description:Gene copy number comparison between similar wine yeast strains of Saccharomyces cerevisiae from different geographic origins.
2010-02-14 | GSE16941 | GEO
Project description:We studied transcriptional responses comparing an Agave strain ITD00185 with a wine strain T73 in a synthetic agave must
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Project description:Gene copy number comparison between similar wine yeast strains of Saccharomyces cerevisiae from different geographic origins. Microarray-CGH of wine strains EC1118, L-957 and LV CB compared to a common reference. Reference DNA was from laboratory strain S288C.
2010-02-13 | E-GEOD-16941 | biostudies-arrayexpress
Project description:In wine fermentation, the blending of non-Saccharomyces yeast with Saccharomyces cerevisiae to improve the complexity of wine has become common practice, but data regarding the impact on yeast physiology and on genetic and metabolic regulation remain limited. Here we describe a transcriptomic analysis of single species and mixed species fermentations.
2019-02-01 | GSE112581 | GEO
Project description:In the search for renewable sources of energy, bioethanol stands out as a benchmark biofuel because its production is based on a proven technological platform. Bioethanol is produced mainly from the fermentation of carbohydrates derived from agricultural feedstocks by the yeast Saccharomyces cerevisiae. One of the most widely adopted strains is PE-2, a heterothallic diploid naturally adapted to the sugar cane fermentation process used in Brazil. Here we report the molecular genetic analysis of a PE-2 derived diploid (JAY270), and the complete genome sequence of a haploid derivative (JAY291). The JAY270 genome is highly heterozygous (~2 SNPs per kilobase), and has several structural polymorphisms between homologous chromosomes. These chromosomal rearrangements are confined to the peripheral regions of the chromosomes, and appear to reflect ectopic homologous recombination between repetitive DNA sequences. Despite the complex karyotype of JAY270, this diploid, when sporulated, had a high frequency of viable spores (~93%). Crosses of haploids derived from JAY270 to a haploid of the unrelated laboratory strain S288c also resulted in diploids that had good spore viability (75-95%). Thus, the rearrangements that exist near the ends of chromosomes do not impair meiosis and spore viability, as they do not span regions that contain essential genes. This observation is consistent with a model in which the peripheral regions of chromosomes represent plastic domains of the genome that are free to recombine ectopically and experiment with alternative structures that may be associated with a fitness benefit. We also explore features of the JAY270 and JAY291 genomes that help explain their high adaptation to industrial environments, exhibiting desirable phenotypes such as high cell mass production and fermentation kinetics, high temperature growth and oxidative stress tolerance. The genomic manipulation of such strains could enable the creation a new generation of industrial organisms, ideally suited for use as delivery vehicles for future bioenergy technologies. This microarray experiment was used to compare the relative gene expression levels between two unrelated S. cerevisiae strain backgrounds: JAY270 and JAY309. Total RNA from each strain was prepared and used to synthesize differentially labeled cDNAs (Cy5 and C3 respectively). A positive Log2 (Red/Green) ratio indicates transcripts more abundant in JAY270, while a negative Log2 (Red/Green) ration indicates transcripts more abundant in JAY309.
2010-05-15 | E-GEOD-17578 | biostudies-arrayexpress
Project description:In the search for renewable sources of energy, bioethanol stands out as a benchmark biofuel because its production is based on a proven technological platform. Bioethanol is produced mainly from the fermentation of carbohydrates derived from agricultural feedstocks by the yeast Saccharomyces cerevisiae. One of the most widely adopted strains is PE-2, a heterothallic diploid naturally adapted to the sugar cane fermentation process used in Brazil. Here we report the molecular genetic analysis of a PE-2 derived diploid (JAY270), and the complete genome sequence of a haploid derivative (JAY291). The JAY270 genome is highly heterozygous (~2 SNPs per kilobase), and has several structural polymorphisms between homologous chromosomes. These chromosomal rearrangements are confined to the peripheral regions of the chromosomes, and appear to reflect ectopic homologous recombination between repetitive DNA sequences. Despite the complex karyotype of JAY270, this diploid, when sporulated, had a high frequency of viable spores (~93%). Crosses of haploids derived from JAY270 to a haploid of the unrelated laboratory strain S288c also resulted in diploids that had good spore viability (75-95%). Thus, the rearrangements that exist near the ends of chromosomes do not impair meiosis and spore viability, as they do not span regions that contain essential genes. This observation is consistent with a model in which the peripheral regions of chromosomes represent plastic domains of the genome that are free to recombine ectopically and experiment with alternative structures that may be associated with a fitness benefit. We also explore features of the JAY270 and JAY291 genomes that help explain their high adaptation to industrial environments, exhibiting desirable phenotypes such as high cell mass production and fermentation kinetics, high temperature growth and oxidative stress tolerance. The genomic manipulation of such strains could enable the creation a new generation of industrial organisms, ideally suited for use as delivery vehicles for future bioenergy technologies.
2009-08-12 | GSE17578 | GEO
Project description:The molecular basis for glucose and xylose fermentation by industrial Saccharomyces cerevisiae is of interest to promote bioethanol production We used microarrays to investigate the transcriptional difference of a industrial strain cultured in both single sugar media and a mixed sugar medium of glucose and xylose
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Project description:In oenological conditions, yeasts are exposed to several stresses and Saccharomyces cerevisiae strains respond modifying their transcriptional program. Yeast flocculation is a social trait that permits to the cells to escape to hostile conditions by sedimentation. This behaviour is mainly due to the presence in the genome of FLO greenbeards genes. In order to understand the role of flocculation in stress response a flocculent S. cerevisiae wine strain and his FLO5 deleted strain were submitted to standard and second fermentation. At the middle of these two processes a genome wide expression analysis was performed. Results showed that while the FLO5 deleted strain was engaged to a re-organization of cell wall and to find a different way to adhere, flocculent yeast presented a proteic stress response and an up-regulation of genes involved in superior alcohols production. Obtained data confirmed the importance of FLO5 in flocculation phenotype and highlight the role of flocculation in stress response.
2017-11-30 | GSE99465 | GEO
Project description:This experiment is the analysis of the transcriptomes of several hybrid yeast strains obtained by crossing natural (from wine) isolates of S. cerevisiae and S. uvarum. All isolations have been done from hybrid strains growing in exponential phase in YPD. Keywords: Strain comparison
2008-07-15 | GSE9888 | GEO
Project description:Natural grape-juice fermentations involve the sequential development of different yeast species which strongly influence the chemical and sensorial traits of the final product. In the present study,we aimed to examine the transcriptomic response of Saccharomyces cerevisiae to the presence of Hanseniaspora guilliermondii wine fermentation.
2015-12-24 | GSE66521 | GEO