Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Danio rerio

ABSTRACT: Autophagy regulator DRAM1 functions downstream of MYD88 in defense against tuberculosis (RNA-seq)

PROVIDER: PRJNA213244 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR942764_1.fastq.gz	Fastqsanger.gz
	SRR942764_2.fastq.gz	Fastqsanger.gz
	SRR942765_1.fastq.gz	Fastqsanger.gz
	SRR942765_2.fastq.gz	Fastqsanger.gz
	SRR942766_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 22

Similar Datasets

Danio rerio

Project description:Autophagy regulator DRAM1 functions downstream of MYD88 in defense against tuberculosis (array)

| PRJNA213245 | ENA

Autophagy regulator DRAM1 functions downstream of MYD88 in defense against tuberculosis

Project description:Autophagy regulator DRAM1 functions downstream of MYD88 in defense against tuberculosis

| PRJNA213243 | ENA

Danio rerio

Project description:Identification of downstream targets of zebrafish Hoxb1a

| PRJNA96559 | ENA

Danio rerio

Project description:Pathogen recognition and activation of the innate immune response in zebrafish: MyD88 mutants

| PRJNA170449 | ENA

Danio rerio

Project description:Intestinal microbiome adjusts the innate immune setpoint during colonization through negative regulation of MyD88

| PRJNA324288 | ENA

Danio rerio

Project description:Autophagy is essential for cardiac morphogenesis during vertebrate development

| PRJNA223395 | ENA

Lysosome repositioning as an autophagy escape mechanism by Mycobacterium tuberuculosis Beijing strain

Project description:Autophagy is a conserved lysosomal-dependent cellular degradation process shown to play a key role in immune defense against Mycobacterium tuberculosis inside host macrophages. Induction of autophagy enhances mycobacterial phagosome acquisition of lysosomal hydrolases, resulting in the destruction of intracellular M. tuberculosis reference strain H37Rv and strains belonging to the East African Indian genotype. However, our previous study showed that strains belonging to the hypervirulent M. tuberculosis Beijing genotype have a special ability to resist autophagic killing but the mechanism involved remains unclear. In this study, we carried out whole transcriptome analyses of host macrophages infected with the autophagy resistant Beijing strain compared to that of H37Rv. Our results identified several genes that are differentially regulated in the Beijing strain-infected host cells including those function in the lysosome positioning pathway. Host macrophages depleted of Kxd1 and Pleckhm2, two proteins in this pathway, can now enhance the lysosomal hydrolase acquisition into the Beijing phagosomes and restrict the bacterial survival upon autophagy induction. High-content image analysis showed an increase in lysosome numbers at the cell periphery in host cells infected with the autophagy resistant Beijing strain in a Pleckhm2-dependent manner. Taken together, these data indicated that the M. tuberculosis Beijing strain escapes autophagic elimination by upregulating the lysosome positioning pathway resulting in an increase in lysosome relocation toward the cell periphery and therefore sparing the mycobacteria from autophagic restriction. Our work thus identified new strategy employed by M. tuberculosis to evade autophagy which may provide potential new targets for drug discovery against tuberculosis

2021-03-02 | GSE151633 | GEO

Danio rerio

Project description:Circadian clock drives novel functions through transcriptional cascade

| PRJNA162305 | ENA

Danio rerio

Project description:The functions of igf2bp3 during zebrafish early development

| PRJNA565584 | ENA

Danio rerio

Project description:Macrophage-specific gene functions in Spi1-directed innate immunity

| PRJNA120815 | ENA