Project description:RNA-seq of Mycobacteriophage Island3 infection of Mycolicibacterium smegmatis mc2155, Mycolicibacterium smegmatis mc2155(Butters), and Mycolicibacterium smegmatis mc2155(Buttersgp57r) to assess the impact of Butters lysogen and specifically Buttersgp57r on transcript levels of island3 during infection.
Project description:We identified a novel transcription factor, MsoA, in response to oxidative stress in Mycolicibacterium smegmatis. We hope to discover the regulatory network and target genes of transcription factor msoA by transcriptome sequencing of wild-type and msoA mutants, so as to help us further elucidate the molecular mechanism of msoA involved in regulation of Mycolicibacterium oxidation.
Project description:We describe a case of catheter-related bacteremia caused by Mycolicibacterium iranicum in the United States. The case highlights the value of using next-generation sequencing to identify infrequent and emerging pathogens and the challenges associated with choosing appropriate treatments because of limited knowledge of drug resistance mechanisms in those emerging pathogens.
