Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Improved Smart-Seq for sensitive full-length transcriptome profiling in single cells

ABSTRACT: Improved Smart-Seq for sensitive full-length transcriptome profiling in single cells

PROVIDER: PRJNA213629 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR944282.fastq.gz	Fastqsanger.gz
	SRR944283.fastq.gz	Fastqsanger.gz
	SRR944284.fastq.gz	Fastqsanger.gz
	SRR944285.fastq.gz	Fastqsanger.gz
	SRR944286.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 76

Similar Datasets

Improved Smart-Seq for sensitive full-length transcriptome profiling in single cells

Project description:Improved Smart-Seq for sensitive full-length transcriptome profiling in single cells.

2013-09-01 | GSE49321 | GEO

Improved Smart-Seq for sensitive full-length transcriptome profiling in single cells

Project description:Improved Smart-Seq for sensitive full-length transcriptome profiling in single cells. Cells of four different origins were profiled using commercial SMARTer and compared to five variants of an improved protocol (Smart-Seq2).

2013-09-01 | E-GEOD-49321 | biostudies-arrayexpress

Scylla Paramamosain Transcriptome (full-length)

Project description:Scylla Paramamosain Transcriptome (full-length)

| PRJEB40077 | ENA

Portunus trituberculatus Transcriptome (full-length)

Project description:Portunus trituberculatus Transcriptome (full-length)

| PRJEB40076 | ENA

Phascolosoma esculenta Transcriptome (full-length)

Project description:Phascolosoma esculenta Transcriptome (full-length)

| PRJEB38426 | ENA

Full-length HLA class II sequences

Project description:Full-length HLA class II sequences

| PRJEB42469 | ENA

Single-cell sequencing of full-length transcripts and T-cell receptors with automated high-throughput Smart-seq3 [10X]

Project description:We developed an automated high-throughput Smart-seq3 (HT Smart-seq3) workflow via robotic implementation and established best practices to consistently achieve high cell capture efficiency and data quality. In comparison with the 10X platform, HT Smart-seq3 analysis of primary CD4+ T-cells demonstrated superior sensitivity in gene detection and similar capability to capture major cellular heterogeneity upon sufficient scaling up. Notably, through T-cell receptor (TCR) reconstruction, HT Smart-seq3 identified more productive pairs of alpha and beta chains without additional primer design, enabling more comprehensive profiling of TCRs. Collectively, HT Smart-seq3 provides a cost-effective and scalable method for characterization of single-cell transcriptomes and immune repertoires.

2024-07-02 | GSE270917 | GEO

Single-cell sequencing of full-length transcripts and T-cell receptors with automated high-throughput Smart-seq3 [Bulk]

2024-06-24 | GSE270511 | GEO

Single-cell full-length RNA-sequencing of MCF7 and BT-474 cells using down-scaled Smart-seq2

Project description:Single-cell full-length RNA-sequencing of MCF7 and BT-474 cells using down-scaled Smart-seq2

| PRJNA831806 | ENA

Single-cell sequencing of full-length transcripts and T-cell receptors with automated high-throughput Smart-seq3 [HT SS3]

2024-07-02 | GSE270928 | GEO