Project description:Organohalide-respiring Dehalococcoidia bacteria are one of the few microorganisms capable of transforming chlorinated solvents to benign ethene in anoxic environments. The tceA gene found in these bacteria, coding the trichloroethene-dechlorinating RDase TceA, is frequently detected in contaminated groundwater but not recognized as a biomarker for vinyl chloride detoxification. Here, we demonstrate that the tceA-carrying Dehalococcoides mccartyi (Dhc) strains FL2 and 195 grow with VC as electron acceptor when sufficient vitamin B12 is provided. Global proteomic profiling confirmed the predominant TceA expression in VC-grown Dhc FL2 cells, providing a line of evidence for the implication of TceA in respiratory VC reductive dechlorination.
Project description:Development of a Fluorescence-Activated Cell Sorting Method Coupled with Whole Genome Amplification To Analyze Minority and Trace Dehalococcoides Genomes in Microbial Communities
Project description:Meta-Analyses of Dehalococcoides mccartyi Strain 195 Transcriptomic Profiles Identify a Respiration Rate-Related Gene Expression Transition Point and Interoperon Recruitment of a Key Oxidoreductase Subunit
Project description:Dehalococcoides mccartyi obligately depends on organohalide respiration for energy conservation and growth. The genome of strain CBDB1 encodes 32 reductive dehalogenases, which enable the reductive dehalogenation of a broad range of halogenated compounds. It is one of the few strains able to respire chlorinated benzenes. The differential transcriptional response of the dehalogenase-encoding and –associated genes to halogenated aromatic compounds has so far not been studied on a genome-wide level. To understand the global transcriptional response to specific halogenated aromatic compounds, we analyzed and compared the transcriptomes during growth with 1,2,3- and 1,2,4-trichlorobenzene (TCB).
